State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.
ACS Appl Mater Interfaces. 2017 Apr 19;9(15):12914-12918. doi: 10.1021/acsami.7b01866. Epub 2017 Apr 6.
Enzymatic carriers have a demonstrated value for chemical reactions and industrial applications. Here, we present a novel kind of inverse opal hydrogel particles as the enzymatic carriers. The particles were negatively replicated from spherical colloidal crystal templates by using magnetic nanoparticles tagged acrylamide hydrogel. Thus, they were endowed with the features of monodispersity, small volume, complete penetrating structure, and controllable motion, which are all beneficial for improving the efficiency of biocatalysis. In addition, due to the ordered porous nanostructure, the inverse opal hydrogel particles were imparted with unique photonic band gaps (PBGs) and vivid structural colors for encoding varieties of immobilized enzymes and for constructing a multienzymes biocatalysis system. These features of the inverse opal hydrogel particles indicate that they are ideal enzymatic carriers for biocatalysis.
酶载体在化学反应和工业应用中具有明显的价值。在这里，我们提出了一种新型的具有反转蛋白石结构的水凝胶颗粒作为酶载体。这些颗粒是通过使用磁性纳米颗粒标记丙烯酰胺水凝胶从球形胶体晶体模板中负复制而来的。因此，它们具有单分散性、小体积、完全穿透结构和可控运动等特点，这都有利于提高生物催化的效率。此外，由于有序的多孔纳米结构，反转蛋白石水凝胶颗粒具有独特的光子带隙（PBG）和生动的结构颜色，可用于编码各种固定化酶，并构建多酶生物催化系统。这些反转蛋白石水凝胶颗粒的特点表明它们是生物催化的理想酶载体。
