Ding Mingde, Zhang Hui, Liu Lei, Liang Ruilan
Department of Gynaecology, Affiliated Hospital of Taishan Medical University, Taian, Shandong 271000, P.R. China.
Department of Pathology, Affiliated Hospital of Taishan Medical University, Taian, Shandong 271000, P.R. China.
Oncol Lett. 2019 Feb;17(2):1531-1536. doi: 10.3892/ol.2018.9786. Epub 2018 Nov 30.
The expression of nitric oxide synthase 1 (NOS1) and adenosine triphosphate-binding cassette sub-family G member 2 (ABCG2) in cervical cancer tissues was investigated. The messenger ribonucleic acid (mRNA) levels of NOS1 and ABCG2 in 40 cervical cancer specimens and 20 normal cervical specimens were detected via reverse transcription-polymerase chain reaction, and the correlation between them was analyzed via Pearsons correlation analysis. The protein expression levels were detected via western blotting. Moreover, the regulatory mode between NOS1 and ABCG2 and the effects on proliferation and apoptosis of cervical cancer cells were analyzed using the lentiviral transfection technique. The mRNA levels of NOS1 and ABCG2 in the cervical cancer group were significantly increased compared with those in the normal cervical control group (P<0.05). There was a positive correlation between NOS1 and ABCG2 mRNA expression levels in cervical cancer tissues (r=1.246, P=0.014). HeLa and C-33A cell lines with relatively high expression levels of NOS1 and ABCG2 were selected for the study. After interference in the NOS1 expression in HeLa and C-33A cells with sh-NOS1, the protein expression of ABCG2 was also decreased. However, the protein expression level of NOS1 remained unchanged after interference in the ABCG2 expression (P<0.05). After interference in the NOS1 expression, the proliferation capacities of HeLa and C-33A cells were significantly decreased, but the apoptosis levels were obviously increased (P<0.05). The mRNA expression of NOS1 and ABCG2 in cervical cancer tissues is significantly increased. NOS1, as an upstream signal regulator of ABCG2, regulates the growth and apoptosis of tumor cells. Both NOS1 and ABCG2 are important proliferation-promoting oncogenes in cervical cancer, which are expected to provide a certain theoretical basis for the treatment of cervical cancer.
研究了一氧化氮合酶1（NOS1）和三磷酸腺苷结合盒亚家族G成员2（ABCG2）在宫颈癌组织中的表达。通过逆转录-聚合酶链反应检测40例宫颈癌标本和20例正常宫颈标本中NOS1和ABCG2的信使核糖核酸（mRNA）水平，并通过Pearson相关分析分析它们之间的相关性。通过蛋白质印迹法检测蛋白质表达水平。此外，使用慢病毒转染技术分析了NOS1和ABCG2之间的调控模式以及对宫颈癌细胞增殖和凋亡的影响。与正常宫颈对照组相比，宫颈癌组中NOS1和ABCG2的mRNA水平显著升高（P<0.05）。宫颈癌组织中NOS1和ABCG2 mRNA表达水平呈正相关（r=1.246，P=0.014）。选择NOS1和ABCG2表达水平相对较高的HeLa和C-33A细胞系进行研究。用sh-NOS1干扰HeLa和C-33A细胞中NOS1的表达后，ABCG2的蛋白质表达也降低。然而，干扰ABCG2表达后，NOS1的蛋白质表达水平保持不变（P<0.05）。干扰NOS1表达后，HeLa和C-33A细胞的增殖能力显著降低，但凋亡水平明显升高（P<0.05）。宫颈癌组织中NOS1和ABCG2的mRNA表达显著增加。NOS1作为ABCG2的上游信号调节因子，调节肿瘤细胞的生长和凋亡。NOS1和ABCG2都是宫颈癌中重要的促增殖癌基因，有望为宫颈癌的治疗提供一定的理论依据。
