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卷曲乳杆菌对宫颈癌细胞Ect1/E6E7的抑制作用及机制研究与预警因素筛查

Inhibitory effect and mechanism of Lactobacillus crispatus on cervical precancerous cells Ect1/E6E7 and screening of early warning factors.

作者信息

Wan B, Wei L J, Tan T M, Qin L, Wang H

机构信息

Gynecologic Tumor Department, Guangxi Medical University Cancer Hospital, 71 Hedi Road, Zhong Shan Street, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.

出版信息

Infect Agent Cancer. 2023 Feb 1;18(1):5. doi: 10.1186/s13027-023-00483-1.

DOI:10.1186/s13027-023-00483-1
PMID:36726132
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9890743/
Abstract

OBJECTIVE

To study the potential mechanism of Lactobacillus crispatus inhibiting cervical squamous intraepithelial lesion (SIL) and screen the early warning factors of SIL.

METHODS

The effects of Lactobacillus crispatus on the proliferation, apoptosis, cross pore migration and invasion and cytokines of cervical precancerous cells Ect1/E6E7 were detected respectively. The effect of Lactobacillus crispatus on the expression of differential proteins screened in Ect1/E6E7 cells were detected by Western blot.

RESULTS

Lactobacillus crispatus significantly inhibited the proliferation, induced apoptosis and inhibited cell migration of Ect1/E6E7 cells in a time-dependent manner (P < 0.05), but had no significant effect on cell invasion. Lactobacillus crispatus significantly promoted the secretion of Th1 cytokines and inhibited the secretion of Th2 cytokines by Ect1/E6E7 cells (P < 0.05). In addition, compared with SiHa cells in the control group, the expression of differential proteins PCNA, ATM, LIG1 and HMGB1 in Ect1/E6E7cells decreased significantly, while the expression of TDG and OGG1 proteins increased significantly (P < 0.05). ABCG2 protein in Ect1/E6E7 cells was slightly higher than that in SiHa cells, but the difference was not statistically significant. What is interesting is that Lactobacillus crispatus significantly inhibited the expression of ABCG2, PCNA, ATM, LIG1, OGG1 and HMGB1 proteins in Ect1/E6E7 cells, and promoted the expression of TDG protein.

CONCLUSIONS

Lactobacillus crispatus may inhibit the function of Ect1/E6E7 cells through multiple pathways and exert the potential to reverse the progression of SIL.

摘要

目的

研究卷曲乳杆菌抑制宫颈鳞状上皮内病变（SIL）的潜在机制，并筛选SIL的预警因素。

方法

分别检测卷曲乳杆菌对宫颈癌前病变细胞Ect1/E6E7的增殖、凋亡、跨膜迁移、侵袭及细胞因子的影响。采用蛋白质免疫印迹法检测卷曲乳杆菌对Ect1/E6E7细胞中筛选出的差异蛋白表达的影响。

结果

卷曲乳杆菌能显著抑制Ect1/E6E7细胞的增殖，诱导其凋亡，并呈时间依赖性抑制细胞迁移（P＜0.05），但对细胞侵袭无显著影响。卷曲乳杆菌可显著促进Ect1/E6E7细胞分泌Th1细胞因子，抑制其分泌Th2细胞因子（P＜0.05）。此外，与对照组SiHa细胞相比，Ect1/E6E7细胞中增殖细胞核抗原（PCNA）、共济失调毛细血管扩张突变蛋白（ATM）、连接酶1（LIG1）和高迁移率族蛋白B1（HMGB1）等差异蛋白的表达显著降低，而胸腺嘧啶DNA糖基化酶（TDG）和8-氧鸟嘌呤DNA糖基化酶1（OGG1）蛋白的表达显著升高（P＜0.05）。Ect1/E6E7细胞中ATP结合盒转运体G2（ABCG2）蛋白略高于SiHa细胞，但差异无统计学意义。有趣的是，卷曲乳杆菌可显著抑制Ect1/E6E7细胞中ABCG2、PCNA、ATM、LIG1、OGG1和HMGB1蛋白的表达，并促进TDG蛋白的表达。

结论

卷曲乳杆菌可能通过多种途径抑制Ect1/E6E7细胞的功能，发挥逆转SIL进展的潜能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/d20b40a202b3/13027_2023_483_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/c0f5b8fb55c7/13027_2023_483_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/7352e37b6e64/13027_2023_483_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/8eb94e6fe50b/13027_2023_483_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/d95f429a3df9/13027_2023_483_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/d20b40a202b3/13027_2023_483_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/c0f5b8fb55c7/13027_2023_483_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/7352e37b6e64/13027_2023_483_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/8eb94e6fe50b/13027_2023_483_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/d95f429a3df9/13027_2023_483_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2f2/9890743/d20b40a202b3/13027_2023_483_Fig5_HTML.jpg

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