CD146促进肺大细胞神经内分泌癌细胞系的迁移和增殖。

Piao Yicui, Guo Hongyu, Qu Zhibo, Zheng Biao, Gao Yong

Department of Critical Care Medicine, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital and Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, Guangdong 518116, P.R. China.

Department of Medical Administration, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China.

Oncol Lett. 2019 Feb;17(2):2075-2080. doi: 10.3892/ol.2018.9830. Epub 2018 Dec 14.

Dysregulated expression of the cell surface protein, CD146, has been implicated in various types of cancer in humans, including in lung cancer. The present study aimed to clarify the mechanism underlying abnormal CD146 expression in human pulmonary large cell neuroendocrine carcinoma (LCNEC) cell lines (NCI-H460 and NCI-H810). The functions of CD146 were investigated by measuring cell migration and viability following CD146 knockdown or overexpression via small interference RNA and plasmid transfection. The findings demonstrated that decreased protein expression of CD146 could inhibit migration and viability in LCNEC cells. Furthermore, CD146 was determined to influence the expression of epithelial-mesenchymal transition markers (epithelial cadherin, vimentin and Snail) and promoted AKT phosphorylation. The present results imply CD146 may function in the migration and proliferation of pulmonary LCNEC cells.

细胞表面蛋白CD146的表达失调与人类多种类型的癌症有关，包括肺癌。本研究旨在阐明人肺大细胞神经内分泌癌（LCNEC）细胞系（NCI-H460和NCI-H810）中CD146异常表达的潜在机制。通过小干扰RNA和质粒转染敲低或过表达CD146后，测量细胞迁移和活力，研究CD146的功能。研究结果表明，CD146蛋白表达降低可抑制LCNEC细胞的迁移和活力。此外，确定CD146会影响上皮-间质转化标志物（上皮钙黏蛋白、波形蛋白和Snail）的表达，并促进AKT磷酸化。目前的结果表明，CD146可能在肺LCNEC细胞的迁移和增殖中发挥作用。