Zhao Zhongsong, Liu Weiwei, Li Jianhua
Digestive System Department, Shandong Provincial Third Hospital, Jinan, Shandong 250000, P.R. China.
Oncol Lett. 2019 Feb;17(2):2215-2220. doi: 10.3892/ol.2018.9834. Epub 2018 Dec 14.
MicroRNAs (miRNAs) have been reported to influence the occurrence and development of colorectal carcinoma (CRC). In the current research, we explored miR-140-5p function in CRC. In addition, the molecular mechanisms of miR-140-5p/SOX4 axis for CRC were investigated. The miR-140-5p and SOX4 expression levels were evaluated via RT-qPCR or western blot analysis. The MTT and Transwell assay assessed cell proliferation and invasion. Luciferase assay confirmed that miR-140-5p directly targeted SOX4. Moreover, the results showed that miR-140-5p expression was obviously declined in CRC. The low expression of miR-140-5p was related to tumor stage or metastasis. The miR-140-5p overexpression suppressed cell proliferation and invasion in CRC. Moreover, miR-140-5p was identified to directly target SOX4 and the SOX4 expression was increased in CRC. In addition, the high expression of SOX4 promoted cell proliferation and invasion in CRC. The inhibiting effect of miR-140-5p on SOX4 was identified for CRC, and it inhibited the proliferation and invasion. The novel miR-140-5p/SOX4 axis may provide a new therapy for CRC.
据报道，微小RNA（miRNA）会影响结直肠癌（CRC）的发生和发展。在当前研究中，我们探究了miR-140-5p在结直肠癌中的功能。此外，还研究了miR-140-5p/SOX4轴在结直肠癌中的分子机制。通过RT-qPCR或蛋白质免疫印迹分析评估miR-140-5p和SOX4的表达水平。MTT和Transwell实验评估细胞增殖和侵袭能力。荧光素酶实验证实miR-140-5p直接靶向SOX4。此外，结果显示miR-140-5p在结直肠癌中的表达明显下降。miR-140-5p的低表达与肿瘤分期或转移有关。miR-140-5p过表达抑制了结直肠癌细胞的增殖和侵袭。此外，已确定miR-140-5p直接靶向SOX4，且SOX4在结直肠癌中的表达增加。此外，SOX4的高表达促进了结直肠癌细胞的增殖和侵袭。已确定miR-140-5p对结直肠癌中SOX4的抑制作用，且它抑制了增殖和侵袭。新的miR-140-5p/SOX4轴可能为结直肠癌提供一种新的治疗方法。
