Analytical Chemistry Department, School of Chemistry, College of Science, University of Tehran, Tehran, 1417466191, Iran.
Anal Bioanal Chem. 2019 Feb;411(5):1075-1084. doi: 10.1007/s00216-018-1534-4. Epub 2019 Jan 23.
A sensitive fluorescent chemical nanosensor for the detection of entacapone (EN) in pharmaceutical samples is introduced. EN is a nitrocatechol drug that functions as a selective and reversible inhibitor of catechol-O-methyl transferase and is widely prescribed in the treatment of Parkinson disease. Molecularly imprinting technology and graphene oxide quantum dots (GOQDs) were employed in designing the EN fluorescent nanosensor. GOQDs were embedded into an inorganic polymer while the imprinting process occurred. The synthesized GOQDs-embedded silica molecularly imprinting polymer (SMIP) showed strong fluorescent emission at 450 nm by exciting at 360 nm. The fluorescence intensity of GOQDs-embedded SMIP was quenched effectively by adsorption of EN as a template molecule. The quenching corresponded to EN concentration in a linear range of at least 0.40-6.00 μM with a limit of detection of 0.31 μM. The designed chemical nanosensor was successfully applied to the analysis of entacapone in some pharmaceutical tablets also containing carbidopa and levodopa (RSD 3.8%).
介绍了一种用于检测恩他卡朋（EN）的灵敏荧光化学纳米传感器。EN 是一种硝基儿茶酚类药物，作为儿茶酚-O-甲基转移酶的选择性和可逆抑制剂，广泛用于治疗帕金森病。在设计 EN 荧光纳米传感器时，采用了分子印迹技术和氧化石墨烯量子点（GOQDs）。GOQDs 嵌入到无机聚合物中，同时发生印迹过程。合成的 GOQDs 嵌入的二氧化硅分子印迹聚合物（SMIP）在 360nm 激发下，在 450nm 处表现出强烈的荧光发射。GOQDs 嵌入的 SMIP 的荧光强度通过吸附作为模板分子的 EN 被有效猝灭。猝灭与 EN 浓度在至少 0.40-6.00μM 的线性范围内相对应，检测限为 0.31μM。所设计的化学纳米传感器成功地应用于一些含有卡比多巴和左旋多巴的药物片剂中恩他卡朋的分析（RSD 3.8%）。
