Alonso A, Pionetti C H, Scavini L M, Rodríguez S M, Mouchián K
División Immunidad y Alergia, Hospital de Clínicas José de San Martín, Buenos Aires, Argentina.
Allergol Immunopathol (Madr). 1988 Sep-Oct;16(5):347-52.
The pigeon breeder's disease is a form of extrinsic allergic alveolitis caused by the exposure to pigeon droppings. Chemical analysis of this antigen was carried out employing column fractionation techniques. Sephadex G-50 and DEAE cellulose were performed and several proteins and hexoses peaks were recorded. Molecular weights were determined by comparison with standardized marker proteins passed through a drop counting fraction collector. The whole extract revealed a molecular weight of 100 Kd while fraction 1 showed 66 Kd and fraction 2 only 30 Kd. A guinea pig experimental model was developed with the whole extract injected by the intradermal route, administered by an intragastric catheter or aerosolized in a glass chamber. Histopathological studies were carried out with the lungs, kidneys, liver and spleen obtained by the necropsy of the animals. The lungs and the kidneys showed the paramount changes in their structures with lymphomononuclear infiltrates and an Arthus-like phenomenon surrounding the vessels. Immunological techniques were applied to the sera and the lymphocytes obtained from the animals. Precipitin and hemagglutinating IgG antibodies were detected against the whole extract and the fractions obtained by column fractionation. Sensitized lymphocytes were also detected. This experimental model represents a guide in the approach to the human allergic alveolitis whose immunological findings will be presented in a forth-coming report.
鸽饲养者病是一种因接触鸽粪而引起的外源性过敏性肺泡炎。采用柱分级分离技术对该抗原进行了化学分析。使用了葡聚糖G - 50和二乙氨基乙基纤维素，并记录了几个蛋白质和己糖峰。通过与经液滴计数分级收集器的标准化标记蛋白比较来确定分子量。整个提取物显示分子量为100千道尔顿，而组分1显示为66千道尔顿，组分2仅为30千道尔顿。通过皮内注射整个提取物、经胃导管给药或在玻璃腔室内雾化建立了豚鼠实验模型。对通过动物尸检获得的肺、肾、肝和脾进行了组织病理学研究。肺和肾的结构出现了最主要的变化，有淋巴细胞单核细胞浸润以及血管周围类似阿瑟斯反应的现象。对从动物身上获得的血清和淋巴细胞应用了免疫学技术。检测到针对整个提取物和通过柱分级分离获得的组分的沉淀素和血凝IgG抗体。还检测到了致敏淋巴细胞。该实验模型为人类过敏性肺泡炎的研究方法提供了指导，其免疫学研究结果将在即将发表的报告中呈现。
