频率依赖的脂质筏在大鼠膈肌运动终板处的摄取。
Frequency-dependent lipid raft uptake at rat diaphragm muscle axon terminals.
机构信息
Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, Minnesota, USA.
School of Biomedical Sciences, The University of Queensland, St. Lucia, Queensland, Australia.
出版信息
Muscle Nerve. 2019 May;59(5):611-618. doi: 10.1002/mus.26421. Epub 2019 Feb 5.
Abstract
INTRODUCTION
In motor neurons, cholera toxin B (CTB) binds to the cell-surface ganglioside GM1 and is internalized and transported via structurally unique components of plasma membranes (lipid rafts).
METHODS
Lipid raft uptake by axon terminals adjoining type-identified rat diaphragm muscle fibers was investigated using CTB and confocal imaging.
RESULTS
Lipid raft uptake increased significantly at higher frequency stimulation (80 Hz), compared with lower frequency (20 Hz) and unstimulated (0 Hz) conditions. The fraction of axon terminal occupied by CTB was ∼45% at 0- or 20-Hz stimulation, and increased to ∼65% at 80 Hz. Total CTB fluorescence intensity also increased (∼20%) after 80-Hz stimulation compared with 0 Hz.
DISCUSSION
Evidence of increased lipid raft uptake at high stimulation frequencies supports an important role for lipid raft signaling at rat diaphragm muscle axon terminals, primarily for motor units physiologically activated at the higher frequencies. Muscle Nerve 59:611-611, 2019.
摘要
简介
在运动神经元中，霍乱毒素 B（CTB）与细胞表面神经节苷脂 GM1 结合，并通过质膜（脂质筏）的独特结构成分内化和运输。
方法
使用 CTB 和共聚焦成像研究了与鉴定的大鼠膈肌纤维相邻的轴突末梢的脂质筏摄取。
结果
与低频（20 Hz）和未刺激（0 Hz）相比，高频（80 Hz）刺激时脂质筏摄取显著增加。在 0 或 20 Hz 刺激时，CTB 占据轴突末梢的比例约为 45%，而在 80 Hz 时增加到约 65%。与 0 Hz 相比，80 Hz 刺激后 CTB 的总荧光强度也增加了约 20%。
讨论
高频刺激时脂质筏摄取增加的证据表明，脂质筏信号在大鼠膈肌运动神经元轴突末梢中具有重要作用，主要是为高频生理性激活的运动单位提供作用。肌肉神经 59:611-611, 2019。
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