Kartsev N N, Svetoch E A, Ershova M G, Abrosimova G N, Tazina O I, Pinchuk A S, Fursova N K, Shepelin A P, Dyatlov I A
The Federal Budget Institution of Science "The State Scientific Center of Applied Microbiology and Biotechnology" of Rospotrebnadzor, 142279, village Obolensk of the Moscow Oblast, Russia.
The State Budget Institution of Health Care "The Yaroslavl oblast infection clinical hospital № 1", Yaroslavl, Russia.
Klin Lab Diagn. 2018;63(4):249-253. doi: 10.18821/0869-2084-63-4-249-253.
The diarrheagenic bacteria coli take a significant place among agents of acute intestinal infections in children aged under 5 years. The main danger among these pathogens is represented by both enterotoxigenic E. coli causing enteritis and enterocolitis accompanied by acute dehydration diarrhea and Escherichia producing shiga-toxin being agents of hemorrhagic colitis and hemolytic uremic syndrome. The fast and proper identification of agents of these two groups of pathogens is an important task of bacteriologists to be resolved for successful treatment of patient because tactics of therapy of enterotoxigenic diarrhea and hemorrhagic colitis and hemolytic uremic syndrome significantly differ. The high capacity of Escherichia coli to form populations resistant to anti-microbial medications, including pan-resistant ones, also is a serious problem for science and public health. The object of study was a collection of isolates of E. coli (n = 112), separated from 112 children aged under 5 years with clinical manifestations of acute intestinal infection, food toxic infection hemocolitis and diarrhea of obscure etiology in Yaroslavl in 2015-2017. Initially, the strains of E. coli were tested using diagnostic agglutinating coli-serums and then using reagents' kit «AmpliSens®Escherichiosis-FL» for detection and differentiation DNAof diarrheagenic bacteria coli and also with specific oligonucleotide primers to genes of virulence and O-serum group belonging. The obtained data permitted to determine belonging of analyzed strains of E. coli to four sub-groups: ЕНЕС (n = 9), EPEC (n = 17), ETEC (n = 1) и EAgEС (n = 1). All of them were agents of genes of pathogenicity specific for every pathogroup. The most numerous group EPEC was represented by strains of five serogroups with dominating among them serogroup O26 (9 strains). Therefore, studying collection of strains of diarrheagenic Escherichia isolated during 2015-1017 in Yaroslavl from children aged under 5 years with acute intestinal infections permitted to demonstrate efficiency of application of molecular genetic methods of analysis for characterizing E. coli i.e. establishment their serogroups, detection of genes of virulence and attributing to pathogroups.
致泻性大肠杆菌在5岁以下儿童急性肠道感染病原体中占据重要地位。这些病原体中的主要危险包括引起肠炎和伴有急性脱水腹泻的小肠结肠炎的产肠毒素大肠杆菌，以及作为出血性结肠炎和溶血尿毒综合征病原体的产志贺毒素大肠杆菌。快速准确地鉴定这两类病原体对于细菌学家来说是一项重要任务，因为产肠毒素腹泻、出血性结肠炎和溶血尿毒综合征的治疗策略有显著差异，这一任务的解决有助于患者的成功治疗。大肠杆菌形成对抗菌药物（包括全耐药菌）耐药菌群的能力很强，这对科学和公共卫生来说也是一个严重问题。研究对象是2015年至2017年在雅罗斯拉夫尔从112名5岁以下患有急性肠道感染、食物中毒性感染、出血性结肠炎和病因不明腹泻临床表现的儿童中分离出的大肠杆菌分离株集合（n = 112）。最初，使用诊断性凝集大肠杆菌血清对大肠杆菌菌株进行检测，然后使用试剂套件“AmpliSens®大肠杆菌病 - FL”检测和区分致泻性大肠杆菌的DNA，还使用针对毒力基因和O血清群归属的特异性寡核苷酸引物进行检测。所获得的数据使得能够确定所分析的大肠杆菌菌株属于四个亚组：肠出血性大肠杆菌（EHEC，n = 9）、肠致病性大肠杆菌（EPEC，n = 17）、产肠毒素大肠杆菌（ETEC，n = 1）和肠集聚性大肠杆菌（EAgEC，n = 1）。它们都是每个致病组特有的致病基因的病原体。数量最多的EPEC组由五个血清群的菌株代表，其中O26血清群占主导（9株）。因此，研究2015 - 2017年在雅罗斯拉夫尔从5岁以下患有急性肠道感染的儿童中分离出的致泻性大肠杆菌菌株集合，证明了应用分子遗传学分析方法来表征大肠杆菌的有效性，即确定它们的血清群、检测毒力基因并归属致病组。
