Cadrin M, Wasteneys G O, Jones-Villeneuve E M, Brown D L, Reuhl K R
Department of Biology, University of Ottawa, Ontario, Canada.
Cell Biol Toxicol. 1988 Mar;4(1):61-80. doi: 10.1007/BF00141287.
Immunofluorescence staining with antibodies to tubulin, neurofilaments and glial filaments was used to study the effects of methylmercury on the differentiation of retinoic acid-induced embryonal carcinoma cells into neurons and astroglia and on the cytoskeleton of these neuroectodermal derivatives. Methylmercury did not prevent undifferentiated embryonal carcinoma cells from developing into neurons and glia. Treatment of committed embryonal carcinoma cells with methylmercury doses exceeding 1 microM resulted in the formation of neurons with abnormal morphologies. In differentiated cultures, microtubules were the first cytoskeletal element to be affected. Their disassembly was time- and concentration-dependent. Microtubules in glial cells and in neuronal perikarya were more sensitive than those in neuronal processes. Neurofilaments and glial filaments appeared relatively insensitive to methylmercury treatment but showed reorganization after complete disassembly of the microtubules. The data demonstrate 1) the sensitivity of microtubules of both neurons and glia to methylmercury-induced depolymerization, and 2) the heterogeneous response of neuronal microtubules to methylmercury, presumably reflecting posttranslational modifications of different subpopulations of microtubules in the perikarya and neurite.
用抗微管蛋白、神经丝和胶质丝的抗体进行免疫荧光染色，以研究甲基汞对视黄酸诱导的胚胎癌细胞分化为神经元和星形胶质细胞的影响，以及对这些神经外胚层衍生物细胞骨架的影响。甲基汞并未阻止未分化的胚胎癌细胞发育为神经元和神经胶质细胞。用超过1微摩尔的甲基汞剂量处理定向的胚胎癌细胞会导致形成形态异常的神经元。在分化的培养物中，微管是第一个受到影响的细胞骨架成分。它们的解聚具有时间和浓度依赖性。胶质细胞和神经元胞体中的微管比神经元突起中的微管更敏感。神经丝和胶质丝对甲基汞处理相对不敏感，但在微管完全解聚后会出现重组。数据表明：1）神经元和胶质细胞的微管对甲基汞诱导的解聚敏感；2）神经元微管对甲基汞的反应具有异质性，这可能反映了胞体和神经突中不同微管亚群的翻译后修饰。
