甲基汞和某些金属离子对小鼠胶质瘤细胞微管网络及体外微管蛋白聚合的影响。

Effects of methylmercury and some metal ions on microtubule networks in mouse glioma cells and in vitro tubulin polymerization.

作者信息

Miura K, Inokawa M, Imura N

出版信息

Toxicol Appl Pharmacol. 1984 Apr;73(2):218-31. doi: 10.1016/0041-008x(84)90327-2.

Abstract

Mercury compounds and some other metal ions were investigated with respect to their effect on in vitro tubulin polymerization and on cellular microtubules in mouse glioma. In vitro tubulin polymerization was completely inhibited by 2.5 X 10(-5) M Hg2+, 5 X 10(-5) M CH3Hg+, 2 X 10(-4) M Cr3+, 2.5 X 10(-4) M Cu2+, and 5 X 10(-4) M Cd2+. Zn2+ did not affect the polymerization up to 5 X 10(-4) M. Indirect immunofluorescence study with rabbit antiporcine tubulin antibody revealed that methylmercury disrupted the microtubule network at an early stage of growth inhibition. On the other hand, in the presence of Cd2+, Cu2+, and Cr3+ at their growth inhibitory concentrations, no effects on microtubule networks were observed for the first 1 hr. These results indicate that only methylmercury affects cellular microtubules, while other ions seem to interfere with other sites in the cells, although these ions showed the ability to depress in vitro tubulin polymerization.

摘要

研究了汞化合物和其他一些金属离子对体外微管蛋白聚合以及小鼠胶质瘤细胞微管的影响。体外微管蛋白聚合被2.5×10⁻⁵ M Hg²⁺、5×10⁻⁵ M CH₃Hg⁺、2×10⁻⁴ M Cr³⁺、2.5×10⁻⁴ M Cu²⁺和5×10⁻⁴ M Cd²⁺完全抑制。高达5×10⁻⁴ M的Zn²⁺不影响聚合。用兔抗猪微管蛋白抗体进行的间接免疫荧光研究表明，甲基汞在生长抑制早期破坏了微管网络。另一方面，在Cd²⁺、Cu²⁺和Cr³⁺处于其生长抑制浓度时，最初1小时未观察到对微管网络有影响。这些结果表明，只有甲基汞影响细胞微管，而其他离子似乎干扰细胞中的其他位点，尽管这些离子显示出抑制体外微管蛋白聚合的能力。