Wasteneys G O, Cadrin M, Reuhl K R, Brown D L
Department of Biology, University of Ottawa, Ontario, Canada.
Cell Biol Toxicol. 1988 Mar;4(1):41-60. doi: 10.1007/BF00141286.
Immunofluorescence staining with antibodies to tubulin and vimentin and staining with phalloidin have been used to examine the effects of methylmercury on the cytoskeleton of embryonal carcinoma cells in culture. Exposure of embryonal carcinoma cells to methylmercury (0.01 to 10 microns) resulted in concentration- and time-dependent disassembly of microtubules in interphase and mitotic cells. These effects were reversible when cultures were washed free of methylmercury. Spindle microtubules were more sensitive than those of interphase cells. Spindle damage resulted in an accumulation of cells in prometaphase/metaphase, which correlated with a temporary delay in the resumption of normal proliferation rate upon removal of methylmercury. Of the interphase cytoskeletal components, microtubules were the first affected by methylmercury. Vimentin intermediate filaments appeared relatively insensitive to methylmercury, but showed a reorganization secondary to the microtubule disassembly. Actin microfilaments appeared unchanged in cells showing complete absence of microtubules. Our results 1) support previous reports suggesting that microtubules are a primary target of methylmercury, 2) document a differential sensitivity of mitotic and interphase microtubule systems and 3) demonstrate the relative insensitivities of other cytoskeletal components.
使用抗微管蛋白和波形蛋白的抗体进行免疫荧光染色以及用鬼笔环肽染色，已用于研究甲基汞对培养的胚胎癌细胞细胞骨架的影响。将胚胎癌细胞暴露于甲基汞（0.01至10微米）会导致间期和有丝分裂细胞中微管的浓度和时间依赖性解聚。当培养物用不含甲基汞的溶液洗涤时，这些影响是可逆的。纺锤体微管比间期细胞的微管更敏感。纺锤体损伤导致细胞在前中期/中期积累，这与去除甲基汞后正常增殖速率的暂时延迟相关。在间期细胞骨架成分中，微管是最先受到甲基汞影响的。波形蛋白中间丝对甲基汞相对不敏感，但在微管解聚后会出现重组。在完全没有微管的细胞中，肌动蛋白微丝似乎没有变化。我们的结果1）支持先前的报道，表明微管是甲基汞的主要靶标，2）记录了有丝分裂和间期微管系统的差异敏感性，3）证明了其他细胞骨架成分的相对不敏感性。
