Cumming R, Burgoyne R D, Lytton N A
Eur J Cell Biol. 1983 Sep;31(2):241-8.
Using two monoclonal antibodies that specifically recognise alpha-tubulin we describe differences in their light and electron microscopic immunoperoxidase staining of axons in cerebellum, hippocampus, and cerebral cortex. In the molecular layer of the cerebellar cortex at the light microscopic level, one of the antibodies (YOL/34) labelled parallel fibre axons (in an identical manner to a beta-tubulin monoclonal antibody) while the other antibody (YL1/2) failed to label them. Extending these studies to the electron microscopic level in the cerebellum we have determined the sub-cellular localisation of alpha-tubulin in microtubules and the postsynaptic density, and also demonstrated a sub-population of parallel fibres and myelinated axons labelled with antibody YL1/2. The monoclonal antibodies were further characterised using immunoblotting against alpha-tubulin separated by isoelectric focusing gels. The results suggest that the contrasting staining patterns between the alpha-tubulin antibodies may reflect axonal sub-populations containing different isotypes of alpha-tubulin.
我们使用两种特异性识别α-微管蛋白的单克隆抗体，描述了它们在小脑、海马体和大脑皮质中对轴突进行光镜和电镜免疫过氧化物酶染色的差异。在小脑皮质分子层的光镜水平上，其中一种抗体（YOL/34）标记平行纤维轴突（方式与β-微管蛋白单克隆抗体相同），而另一种抗体（YL1/2）未能标记它们。将这些研究扩展到小脑的电镜水平，我们确定了α-微管蛋白在微管和突触后致密物中的亚细胞定位，还证明了用抗体YL1/2标记的平行纤维和有髓轴突亚群。使用针对通过等电聚焦凝胶分离的α-微管蛋白的免疫印迹对单克隆抗体进行了进一步表征。结果表明，α-微管蛋白抗体之间形成对比的染色模式可能反映了含有不同α-微管蛋白同种型的轴突亚群。
