Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan.
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Sci Rep. 2019 Jan 24;9(1):573. doi: 10.1038/s41598-018-37224-z.
Recently, bat adenoviruses (BtAdVs) of genus Mastadenovirus have been isolated from various bat species, some of them displaying a wide host range in cell culture. In this study, we isolated two BtAdVs from Japanese wild microbats. While one isolate was classified as Bat mastadenovirus A, the other was phylogenetically independent of other BtAdVs. It was rather related to, but serologically different from, canine adenoviruses. We propose that the latter, isolated from Asian parti-colored bat, should be assigned to a novel species of Bat mastadenovirus. Both isolates replicated in various mammalian cell lines, implying their wide cell tropism. To gain insight into cell tropism of these BtAdVs, we investigated the coxsackievirus and adenovirus receptor (CXADR) for virus entry to the cells. We prepared CXADR-knockout canine kidney cells and found that replication of BtAdVs was significantly hampered in these cells. For confirmation, their replication in canine CXADR-addback cells was rescued to the levels with the original cells. We also found that viral replication was corrected in human or bat CXADR-transduced cells to similar levels as in canine CXADR-addback cells. These results suggest that BtAdVs were able to use several mammalian-derived CXADRs as entry factors.
最近，从各种蝙蝠物种中分离出了属于 Mastadenovirus 属的蝙蝠腺病毒（BtAdVs），其中一些在细胞培养中显示出广泛的宿主范围。在这项研究中，我们从日本野生微型蝙蝠中分离出了两种 BtAdVs。一种分离株被归类为蝙蝠腺病毒 A，另一种与其他 BtAdVs 在系统发育上是独立的。它与犬腺病毒关系密切，但血清学上与犬腺病毒不同。我们提出，后者从亚洲彩色蝙蝠中分离出来，应被分配到一种新的蝙蝠腺病毒物种中。两种分离株均可在多种哺乳动物细胞系中复制，表明其具有广泛的细胞嗜性。为了深入了解这些 BtAdVs 的细胞嗜性，我们研究了柯萨奇病毒和腺病毒受体（CXADR）在病毒进入细胞中的作用。我们制备了 CXADR 敲除犬肾细胞，并发现 BtAdVs 的复制在这些细胞中受到显著阻碍。为了确认这一点，我们在犬 CXADR 添加回细胞中恢复了 BtAdVs 的复制，使其达到与原始细胞相同的水平。我们还发现，在转导了人或蝙蝠 CXADR 的细胞中，病毒复制被纠正到与犬 CXADR 添加回细胞相似的水平。这些结果表明，BtAdVs 能够利用几种哺乳动物衍生的 CXADR 作为进入因子。
