Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
Clin Oral Investig. 2019 Mar;23(3):1489-1493. doi: 10.1007/s00784-019-02805-3. Epub 2019 Jan 24.
This study aimed to define the comprehensive bacterial flora of the healthy oral cavity by identifying and comparing bacterial species in different subgingival sites using 454 sequencing of 16S rRNA genes.
Subgingival plaque samples were taken from six target teeth (central incisor, first premolar, and first molar in both the maxilla and mandible) of 10 periodontally healthy patients. Bacterial DNA was extracted and next-generation sequencing of 16S rRNA genes was performed.
Bacterial composition in phylum level was similar for all sites within the same individual irrespective of tooth location. Unweighted UniFrac distance values of microbiome also showed that average distance was significantly larger between subjects than between tooth locations of the same subjects.
The present results clarify the lack of effect of tooth location in the healthy subgingival microbiota. Results may suggest that any subgingival site can demonstrate similar subject-specific microbiota.
This investigation offers a better understanding of the uniqueness of the oral microbiome. The present study will facilitate sampling in future subgingival microbiological studies.
本研究旨在通过对不同龈下位点的细菌物种进行鉴定和比较，利用 16S rRNA 基因的 454 测序来定义健康口腔的综合细菌菌群。
从 10 名牙周健康患者的 6 颗目标牙齿（上颌和下颌的中切牙、第一前磨牙和第一磨牙）中采集龈下菌斑样本。提取细菌 DNA，进行 16S rRNA 基因的下一代测序。
无论牙齿位置如何，同一个体内所有部位的细菌组成在门水平上相似。微生物组的未加权 UniFrac 距离值也表明，受试者之间的平均距离明显大于同一受试者的牙齿位置之间的距离。
本研究结果阐明了牙齿位置对健康龈下微生物群无影响。结果可能表明，任何龈下部位都可以表现出相似的个体特异性微生物群。
本研究提供了对口腔微生物组独特性的更好理解。本研究将有助于未来龈下微生物学研究中的采样。
