Department of Biology & CESAM, University of Aveiro, Aveiro, Portugal.
PLoS One. 2019 Jan 25;14(1):e0211209. doi: 10.1371/journal.pone.0211209. eCollection 2019.
There is a growing consensus that future technological developments of aquaculture systems should account for the structure and function of microbial communities in the whole system and not only in fish guts. In this study, we aimed to investigate the composition of bacterioplankton communities of a hatchery recirculating aquaculture system (RAS) used for the production of Senegalese sole (Solea senegalensis) juveniles. To this end, we used a 16S rRNA gene based denaturing gradient gel electrophoresis (DGGE) and pyrosequencing analyses to characterize the bacterioplankton communities of the RAS and its water supply. Overall, the most abundant orders were Alteromonadales, Rhodobacterales, Oceanospirillales, Vibrionales, Flavobacteriales, Lactobacillales, Thiotrichales, Burkholderiales and Bdellovibrionales. Although we found a clear distinction between the RAS and the water supply bacterioplankton communities, most of the abundant OTUs (≥50 sequences) in the hatchery RAS were also present in the water supply. These included OTUs related to Pseudoalteromonas genus and the Roseobacter clade, which are known to comprise bacterial members with activity against Vibrio fish pathogens. Overall, in contrast to previous findings for sole grow-out RAS, our results suggest that the water supply may influence the bacterioplankton community structure of sole hatchery RAS. Further studies are needed to investigate the effect of aquaculture practices on RAS bacterioplankton communities and identification of the key drivers of their structure and diversity.
越来越多的人认为，未来水产养殖系统的技术发展应该考虑整个系统中微生物群落的结构和功能，而不仅仅是鱼类肠道中的微生物。在本研究中，我们旨在调查用于生产塞内加尔比目鱼（Solea senegalensis）幼鱼的孵化场循环水产养殖系统（RAS）中浮游细菌群落的组成。为此，我们使用基于 16S rRNA 基因的变性梯度凝胶电泳（DGGE）和 pyrosequencing 分析来表征 RAS 及其供水的浮游细菌群落。总体而言，最丰富的目是交替单胞菌目、红杆菌目、海洋螺旋菌目、振动菌目、黄杆菌目、乳杆菌目、硫杆菌目、伯克霍尔德菌目和贝氏菌目。尽管我们发现 RAS 和供水浮游细菌群落之间存在明显区别，但孵化场 RAS 中大多数丰富的 OTUs（≥50 个序列）也存在于供水中。其中包括与假交替单胞菌属和玫瑰杆菌群相关的 OTUs，这些 OTUs通常包含具有抗弧菌鱼类病原体活性的细菌成员。总的来说，与之前对 sole 养殖 RAS 的研究结果相反，我们的结果表明，供水可能会影响 sole 孵化场 RAS 的浮游细菌群落结构。需要进一步的研究来调查水产养殖实践对 RAS 浮游细菌群落的影响，并确定其结构和多样性的关键驱动因素。
