Sowa Sylwia, Paczos-Grzęda Edyta, Koroluk Aneta, Okoń Sylwia, Ostrowska Agnieszka, Ociepa Tomasz, Chrząstek Maria, Kowalczyk Krzysztof
Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Lublin, Poland.
Plant Dis. 2016 Jun;100(6):1184-1191. doi: 10.1094/PDIS-06-15-0671-RE. Epub 2016 Mar 22.
Wild oat tetraploids of the section Pachycarpa have already been proven to be a rich source of useful genes but have largely been unexploited for Puccinia coronata resistance. In this study, accessions of Avena magna, A. murphyi, and A. insularis gathered from European and North American gene banks were evaluated at the seedling stage for crown rust reaction using the host-pathogen test and six highly diverse and virulent P. coronata isolates. Of the 92 Avena accessions analyzed, 58.7% were resistant to at least one crown rust race. In all, 37% of the tested accessions reacted nonuniformly, which indicated their heterogeneity. The highest level of resistance was observed in three of the accessions, one of which was verified by flow cytometry as being hexaploid and two of which were verified as being tetraploids. The infection profiles of 19 accessions corresponded to resistance determined by the genes Pc14, Pc39, Pc40, Pc48, Pc50, Pc54, Pc55, Pc61, Pc67, Pc68, Pc97, Pc101, or Pc104. The patterns of infection of the remaining resistant A. magna and A. murphyi accessions allowed us to postulate the presence of potentially novel crown rust resistance genes.
厚果燕麦组的野生燕麦四倍体已被证明是有用基因的丰富来源，但在抗冠锈病方面基本上未被开发利用。在本研究中，从欧洲和北美基因库收集的大燕麦、墨菲燕麦和海岛燕麦种质在幼苗期使用寄主-病原菌试验和六种高度多样且具毒性的冠锈菌分离株进行了冠锈病反应评估。在分析的92份燕麦种质中，58.7%对至少一种冠锈病小种具有抗性。总体而言，37%的测试种质反应不一致，这表明它们具有异质性。在其中三份种质中观察到了最高水平的抗性，其中一份经流式细胞术验证为六倍体，两份经验证为四倍体。19份种质的感染情况与由Pc14、Pc39、Pc40、Pc48、Pc50、Pc54、Pc55、Pc61、Pc67、Pc68、Pc97、Pc101或Pc104基因决定的抗性相对应。其余具抗性的大燕麦和墨菲燕麦种质的感染模式使我们推测可能存在新的冠锈病抗性基因。
