CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience , National Center for Nanoscience and Technology , Beijing 100190 , China.
University of Chinese Academy of Sciences , 19 A Yuquan Rd , Shijingshan District, Beijing 100049 , China.
ACS Chem Neurosci. 2019 Mar 20;10(3):1204-1208. doi: 10.1021/acschemneuro.8b00540. Epub 2019 Jan 29.
Parkinson's disease (PD) is a severe neurodegenerative disease and there is great need for developing a biochemical detection method to precisely diagnose it. Alpha-synuclein (α-syn) participates in the main pathology of PD and serves as an important biomarker of PD. Here, we identified peptoid ASBP-7 that had high affinity and specificity to α-syn by screening a peptoid library using the high-throughput surface plasmon resonance imaging method. We confirmed that ASBP-7 could significantly distinguish PD sera from the normal ones through identifying α-syn in the serum. We also demonstrated the high sensitivity of this system in detecting PD serum. This work provides a method for the blood-based, label-free, high-throughput analysis of PD serum, and holds great potential for the early diagnosis and dynamic monitoring of PD.
帕金森病（PD）是一种严重的神经退行性疾病，因此非常需要开发一种生化检测方法来准确诊断它。α-突触核蛋白（α-syn）参与 PD 的主要病理过程，是 PD 的重要生物标志物。在这里，我们通过使用高通量表面等离子体共振成像方法筛选肽库，鉴定出了对 α-syn 具有高亲和力和特异性的肽 ASBP-7。我们通过鉴定血清中的 α-syn，证实了 ASBP-7 可以显著区分 PD 血清和正常血清。我们还证明了该系统在检测 PD 血清方面具有很高的灵敏度。这项工作为基于血液的、无标记的、高通量的 PD 血清分析提供了一种方法，对于 PD 的早期诊断和动态监测具有很大的潜力。
