School of Pharmacy, Weifang Medical University, Weifang 261053, China.
Tianjin Key Laboratory for Photoelectric Materials and Devices, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China.
Molecules. 2022 May 6;27(9):2978. doi: 10.3390/molecules27092978.
In this paper, we designed and synthesized a novel phenylazo-based fluorescent probe (RHN) for the sensing and imaging of hypochlorous acid (HClO) in mitochondria in living cells. In this process, HClO promoted the oxidation of the phenylazo group to generate a free Rhodol fluorophore moiety, which in turn restored strong fluorescence and realized the detection of HClO. As expected, RHN exhibited high selectivity, high sensitivity and rapid response, with detection limits as low as 22 nM (1.155 ng/mL). Importantly, the results of the cell imaging experiments indicated that RHN has the ability to image and sense HClO in mitochondria, which is of great significance for exploration of the specific role of HClO in both the immune system and diseases.
在本文中，我们设计并合成了一种新型基于苯偶氮的荧光探针（RHN），用于检测和成像活细胞线粒体中的次氯酸（HClO）。在这个过程中，HClO 促进了苯偶氮基团的氧化，生成游离的若丹明荧光团部分，从而恢复了强荧光，并实现了 HClO 的检测。正如预期的那样，RHN 表现出高选择性、高灵敏度和快速响应，检测限低至 22 nM（1.155ng/mL）。重要的是，细胞成像实验的结果表明，RHN 具有成像和检测线粒体中 HClO 的能力，这对于探索 HClO 在免疫系统和疾病中的特定作用具有重要意义。
