Kanwichai S, Panasophonkul S, Vos P L A M, Suriyasathaporn W
Department of Food Animal Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
Department of Companion Animal and Wildlife Clinic, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.
Trop Anim Health Prod. 2019 Jun;51(5):1279-1282. doi: 10.1007/s11250-019-01800-w. Epub 2019 Jan 25.
The aims of this study were to determine the effects of seasonality on yields and quality of bovine abattoir-derived oocytes and their in vitro maturation (IVM) competence in Thailand, a tropical country. Ovaries were collected monthly from a slaughterhouse, from January to June 2017, separated into cool (January to February), summer (March to April), and rainy (May to June) seasons. Cumulus oocyte complexes (COCs) were obtained by follicular aspiration, and subsequently, oocyte yield and quality were examined. Selected class I and II COCs were cultured under IVM condition, and their maturation capacity was also evaluated. Results showed that average numbers of COCs and class I COCs per ovary were highest in summer. However, no significant seasonal difference in oocyte maturation rates was found. The higher numbers of COCs and class I COCs in summer might be caused by their follicular development during the cool season, and might be a reason why the pregnancy rate in summer is better than in the rainy season.
本研究的目的是在热带国家泰国，确定季节变化对源自牛屠宰场的卵母细胞的产量和质量及其体外成熟（IVM）能力的影响。2017年1月至6月，每月从一家屠宰场收集卵巢，分为凉爽季节（1月至2月）、夏季（3月至4月）和雨季（5月至6月）。通过卵泡抽吸获得卵丘卵母细胞复合体（COC），随后检查卵母细胞的产量和质量。选择I级和II级COC在IVM条件下培养，并评估其成熟能力。结果显示，每个卵巢的COC和I级COC的平均数量在夏季最高。然而，未发现卵母细胞成熟率存在显著的季节差异。夏季COC和I级COC数量较多可能是由于它们在凉爽季节的卵泡发育，这可能是夏季妊娠率高于雨季的一个原因。
