一种易于操作的高通量植物基因组 DNA 提取方法。

An easily-performed high-throughput method for plant genomic DNA extraction.

机构信息

Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, China; Henan Key Laboratory of Crop Molecular Breeding & Bioreactor, Zhoukou, 466001, China.

Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, China; Henan Key Laboratory of Crop Molecular Breeding & Bioreactor, Zhoukou, 466001, China; College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, China.

出版信息

Anal Biochem. 2019 Mar 15;569:28-30. doi: 10.1016/j.ab.2019.01.007. Epub 2019 Jan 25.

DOI:10.1016/j.ab.2019.01.007

PMID:30685237

Abstract

Genomic DNA isolation is a crucial technique for researchers studying plant molecular biology. A current widely-used protocol for DNA extraction needs a pestle and mortal for each sample and consumes a large amount of liquid nitrogen in grinding the samples. Most high-throughput methods depend on expensive machines for tissue homogenization. Here we developed a CTAB-based DNA extraction method using 2.0 mL microcentrifuge tubes for sample processing. This protocol has the advantages that it is suitable for a variety of plants, easily-performed without special equipment, and high-throughput; it effectively avoids sample cross-contamination, and is inexpensive, rapid and safe.

摘要

基因组 DNA 分离是研究植物分子生物学的研究人员的关键技术。目前广泛使用的 DNA 提取方案需要每个样品一个研钵和杵，并在研磨样品时消耗大量液氮。大多数高通量方法都依赖于昂贵的组织匀浆机。在这里，我们开发了一种基于 CTAB 的 DNA 提取方法，使用 2.0 mL 微量离心管进行样品处理。该方案具有以下优点：适用于各种植物，无需特殊设备即可轻松进行，高通量；它有效地避免了样品交叉污染，并且经济、快速和安全。