Chen Xuyu, Yang Yun, Liu Yangyang, Sui Chun, Wei Jianhe
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & Peking Union Medical College, Haikou 570311, China.
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Chin Herb Med. 2025 Feb 11;17(2):315-321. doi: 10.1016/j.chmed.2025.02.001. eCollection 2025 Apr.
The objective of this study was to analyse fungal composition and exploit application potential in the Bantou (BT) agarwood-forming trunk of
BT agarwood is a naturally formed agarwood that was collected after cutting. Total genomic DNA of the fungi in BT agarwood was extracted by the hexadecyltrimethy ammonium bromide (CTAB) method, followed by PCR amplification and library construction. The effective tags were obtained by the HiSeq2500 platform, and the data were subjected to bioinformatics and statistical analyses.
A total of 7 850 040 effective tags were obtained, Ascomycota was the most abundant fungus at the phylum level, with a relative abundance of 56.36%-61.44%, followed by Basidiomycota, with a relative abundance of 10.49%-20.39%. Dothideomycetes, Agaricomycetes and Sordariomycetes were dominant at the class level, accounting for 26.21%-33.88%, 8.40%-17.66%, and 18.41%-24.11%, respectively. , and were dominant at the genus level, with relative abundances of 6.25%-7.64%, 1.95%-9.05% and 1.5%-5.4%, respectively. Diversity and richness analysis showed that the fungal composition in the agarwood formation sites (agarwood layer, upper agarwood layer and lower agarwood layer) were significantly lower than those in the decomposing layer and the healthy layer. That is, the fungal diversity and richness were significantly reduced during agarwood formation by the action of open wounds. The fungal community structure in the decomposing layer and agarwood formation sites obviously differed from that in the healthy layer. The number of taxa in agarwood formation sites decreased significantly (healthy layer is 0.5%, decomposing layer is 0.022%, upper agarwood layer is 0.012%, agarwood layer is 0.01%, and lower agarwood layer is 0.013%), indicating that agarwood may contain potential substances to inhibit the growth of .
Agarwood from agarwood formation sites contains potential substances that inhibit , which provides valuable information for the control of the genus of .
本研究旨在分析汕头（BT）沉香形成树干中的真菌组成并挖掘其应用潜力。
BT沉香是一种天然形成的沉香，砍伐后采集。采用十六烷基三甲基溴化铵（CTAB）法提取BT沉香中真菌的总基因组DNA，随后进行PCR扩增和文库构建。通过HiSeq2500平台获得有效标签，并对数据进行生物信息学和统计分析。
共获得7850040个有效标签，在门水平上，子囊菌门是最丰富的真菌，相对丰度为56.36% - 61.44%，其次是担子菌门，相对丰度为10.49% - 20.39%。座囊菌纲、伞菌纲和粪壳菌纲在纲水平上占主导地位，分别占26.21% - 33.88%、8.40% - 17.66%和18.41% - 24.11%。[此处原文缺失三个属名]在属水平上占主导地位，相对丰度分别为6.25% - 7.64%、1.95% - 9.05%和1.5% - 5.4%。多样性和丰富度分析表明，沉香形成部位（沉香层、上沉香层和下沉香层）的真菌组成明显低于腐烂层和健康层。也就是说，在沉香形成过程中，由于开放性伤口的作用，真菌多样性和丰富度显著降低。腐烂层和沉香形成部位的真菌群落结构明显不同于健康层。沉香形成部位的[此处原文缺失一个菌属名]类群数量显著减少（健康层为0.5%，腐烂层为0.022%，上沉香层为0.012%，沉香层为0.01%，下沉香层为0.013%），表明沉香可能含有抑制[此处原文缺失一个菌属名]生长的潜在物质。
沉香形成部位的沉香含有抑制[此处原文缺失一个菌属名]的潜在物质，这为控制[此处原文缺失一个菌属名]属提供了有价值的信息。
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