College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.
Mikrochim Acta. 2021 Feb 3;188(3):63. doi: 10.1007/s00604-021-04719-y.
A photothermal immunoassay using a thermometer as readout based on polyaniline@Au organic-inorganic nanohybrids was built. Temperature output is acquired due to the photothermal effect of the photothermal nanomaterial. Polyaniline@Au organic-inorganic nanohybrids were synthesized by interfacial reactions with high photothermal conversion efficiency. A sandwich structure of the immunocomplex was prepared on a microplate for determination of carcinoembryonic antigen (CEA) by polyaniline@Au organic-inorganic nanohybrids as nanolabel. The released heat based on light-to-heat conversion from the photothermal nanolabel under NIR irradiation is detectable using the thermometer. The increased temperature is directly proportional to CEA concentration. The linear range of the photothermal immunoassay is 0.20 to 25 ng mL with determination limit of 0.17 ng mL. Polyaniline@Au organic-inorganic nanohybrids with high photothermal conversion efficiency was synthesized as labels to construct photothermal immunosensor. The sandwich-type immunoassay was built on 96 hole plate based on specific binding of antigen and antibody. Carcinoembryonic antigen in sample was detected quantitatively by thermometer readout.
基于聚苯胺@金有机-无机纳米杂化材料的温度计读出光热免疫分析方法构建。由于光热纳米材料的光热效应而获得温度输出。通过具有高光热转换效率的界面反应合成了聚苯胺@金有机-无机纳米杂化材料。在微孔板上制备免疫复合物的三明治结构，通过聚苯胺@金有机-无机纳米杂化材料作为纳米标记物来测定癌胚抗原（CEA）。在近红外照射下，光热纳米标记物的光热转换产生的释放热量可以用温度计检测到。增加的温度与 CEA 浓度成正比。光热免疫分析的线性范围为 0.20 至 25ngmL，检测限为 0.17ngmL。合成了具有高光热转换效率的聚苯胺@金有机-无机纳米杂化材料作为标签，构建光热免疫传感器。基于抗原和抗体的特异性结合，在 96 孔板上构建了三明治型免疫分析。通过温度计读数定量检测样品中的癌胚抗原。
