Song Yingying, Zhang Zhengqun, Chen Lele, He Leiming, Lu Hongbao, Ren Yupeng, Mu Wei, Liu Feng
College of Plant Protection, Shandong Agricultural University, Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, Tai'an, Shandong 271018, P.R. China.
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, P.R. China.
Plant Dis. 2016 Jul;100(7):1314-1320. doi: 10.1094/PDIS-10-15-1220-RE. Epub 2016 Apr 22.
Isopyrazam is a new broad-spectrum, foliar-absorbed and -translocated succinate dehydrogenase inhibitor fungicide. In this study, 159 Botrytis cinerea isolates collected from different geographical regions of Shandong Province of China were characterized for baseline sensitivity to isopyrazam. Furthermore, the protective and curative activity of isopyrazam on strawberry fruit and the control efficacy in the field were also determined. In contrast to its mycelial growth, the spore germination of B. cinerea was inhibited completely by lower concentrations of isopyrazam, about 1 μg ml on yeast-peptone-acetate medium. Frequency distributions of isopyrazam 50% effective concentration (EC) values were unimodal curves, with mean EC values of 0.07 ± 0.04 (standard deviation) and 0.68 ± 0.36 μg ml for the inhibition of spore germination and mycelial growth, respectively. In addition, there was no positive multiple resistance between isopyrazam and other classes of botryticides such as diethofencarb, iprodione, pyrimethanil, or SYP-Z048. In field trials conducted during 2014 and 2015, isopyrazam used at a concentration of active ingredient at 150 and 200 g ha provided a control efficacy ranging from 76.7 to 87.8% on leaves and from 81.5 to 90.7% on fruit. These results suggest that isopyrazam has the potential to play an important role in the management of gray mold.
异菌脲是一种新型的广谱、可通过叶片吸收和传导的琥珀酸脱氢酶抑制剂类杀菌剂。在本研究中，对从中国山东省不同地理区域收集的159株灰葡萄孢菌分离株进行了对异菌脲的基线敏感性测定。此外，还测定了异菌脲对草莓果实的保护和治疗活性以及田间防治效果。与菌丝生长情况不同，较低浓度的异菌脲就能完全抑制灰葡萄孢菌的孢子萌发，在酵母蛋白胨乙酸培养基上约1μg/ml即可。异菌脲50%有效浓度（EC）值的频率分布呈单峰曲线，抑制孢子萌发和菌丝生长的平均EC值分别为0.07±0.04（标准差）和0.68±0.36μg/ml。此外，异菌脲与其他类别的杀葡萄孢菌剂如乙霉威、异菌脲、嘧霉胺或SYP-Z048之间不存在正向多重抗性。在2014年和2015年进行的田间试验中，以150和200g/ha的活性成分浓度使用异菌脲，对叶片的防治效果为76.7%至87.8%，对果实的防治效果为81.5%至90.7%。这些结果表明异菌脲在灰霉病管理中具有发挥重要作用的潜力。
