氧敏探头 2P：用于深层组织纵向氧成像的高性能探头。
Oxyphor 2P: A High-Performance Probe for Deep-Tissue Longitudinal Oxygen Imaging.
机构信息
Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Institute of Pharmacology and Toxicology, University of Zurich, Zürich 8057, Switzerland.
出版信息
Cell Metab. 2019 Mar 5;29(3):736-744.e7. doi: 10.1016/j.cmet.2018.12.022. Epub 2019 Jan 24.
Quantitative imaging of oxygen distributions in tissue can provide invaluable information about metabolism in normal and diseased states. Two-photon phosphorescence lifetime microscopy (2PLM) has been developed to perform measurements of oxygen in vivo with micron-scale resolution in 3D; however, the method's potential has not yet been fully realized due to the limitations of current phosphorescent probe technology. Here, we report a new sensor, Oxyphor 2P, that enables oxygen microscopy twice as deep (up to 600 μm below the tissue surface) and with ∼60 times higher speed than previously possible. Oxyphor 2P allows longitudinal oxygen measurements without having to inject the probe directly into the imaged region. As proof of principle, we monitored oxygen dynamics for days following micro-stroke induced by occlusion of a single capillary in the mouse brain. Oxyphor 2P opens up new possibilities for studies of tissue metabolic states using 2PLM in a wide range of biomedical research areas.
组织中氧分布的定量成像可以提供关于正常和患病状态下代谢的宝贵信息。双光子磷光寿命显微镜（2PLM）已被开发用于在 3D 中以微米级分辨率进行体内氧测量；然而，由于当前磷光探针技术的限制，该方法的潜力尚未得到充分实现。在这里，我们报告了一种新的传感器 Oxyphor 2P，它使氧显微镜的深度增加了一倍（达到组织表面以下 600μm），速度提高了大约 60 倍。Oxyphor 2P 允许在不将探针直接注入成像区域的情况下进行纵向氧测量。作为原理验证，我们监测了小鼠大脑中单根毛细血管阻塞引起的微中风后数天的氧动力学。Oxyphor 2P 为使用 2PLM 在广泛的生物医学研究领域研究组织代谢状态开辟了新的可能性。
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