Luts A, Sundler F
Department of Medical Cell Research, University of Lund, Sweden.
Cell Tissue Res. 1989 Nov;258(2):259-67. doi: 10.1007/BF00239446.
The ferret is widely used in functional and neuromorphological studies on the respiratory tract. We have examined the occurrence and distribution of peptide-containing and adrenergic nerve fibers (using dopamine-beta-hydroxylase as a marker). Adrenergic nerve fibers and fibers storing vasoactive intestinal peptide have a widespread distribution along the entire respiratory tract. Adrenergic nerve fibers were found in the lamina propria, as well as around blood vessels and glands and in smooth muscle. Nerve fibers storing vasoactive intestinal peptide occurred in the epithelium, the lamina propria, around blood vessels and glands, and among muscle bundles. Substance P-, neurokinin A- and calcitonin gene-related peptide-containing nerve fibers predominated beneath and within the epithelium along the entire respiratory tract. Neuropeptide Y-containing nerve fibers were prominent among smooth muscle bundles and around glands. The blood vessels in the wall of the airways were richly supplied with peptide-containing nerve fibers and adrenergic fibers. Ganglia located over the outer or dorsal surface of the tracheal wall harbored vasoactive intestinal peptide-containing nerve cell bodies. Substance P and neurokinin A invariably coexisted in the same nerve fibers. Further, coexistence of substance P/neurokinin A and calcitonin gene-related peptide was observed in the nerve fibers associated with the epithelium. Vasoactive intestinal peptide, neuropeptide Y and occasionally also substance P coexisted in the population of nerve fibers associated with blood vessels and smooth muscle. Many adrenergic nerve fibers contained neuropeptide Y.
雪貂被广泛应用于呼吸道的功能和神经形态学研究。我们已研究了含肽神经纤维和肾上腺素能神经纤维的发生及分布情况（以多巴胺-β-羟化酶作为标志物）。肾上腺素能神经纤维和储存血管活性肠肽的纤维沿整个呼吸道广泛分布。在固有层、血管和腺体周围以及平滑肌中均发现了肾上腺素能神经纤维。储存血管活性肠肽的神经纤维出现在上皮、固有层、血管和腺体周围以及肌束之间。P物质、神经激肽A和降钙素基因相关肽的含神经纤维在整个呼吸道上皮下方及上皮内占主导。含神经肽Y的神经纤维在平滑肌束之间和腺体周围较为突出。气道壁中的血管富含含肽神经纤维和肾上腺素能纤维。位于气管壁外表面或背表面的神经节含有储存血管活性肠肽的神经细胞体。P物质和神经激肽A总是共存于同一神经纤维中。此外，在与上皮相关的神经纤维中观察到P物质/神经激肽A和降钙素基因相关肽共存。血管活性肠肽、神经肽Y以及偶尔还有P物质共存于与血管和平滑肌相关的神经纤维群体中。许多肾上腺素能神经纤维含有神经肽Y。
