使用抗体功能化肽纳米管进行病毒检测。
Virus assay using antibody-functionalized peptide nanotubes.
作者信息
MacCuspie Robert I, Banerjee Ipsita A, Pejoux Christophe, Gummalla Sanjay, Mostowski Howard S, Krause Philip R, Matsui Hiroshi
机构信息
Department of Chemistry, City University of New York, Huner College and the Graduate Center, 695 Park Ave, New York, NY, 10065, USA; ; Tel: +1 (212) 650 3918.
Food & Drug Administration, Center for Biologics Evaluation and Research, Office of Vaccine Research and Review, Division of Viral Products, 29 Lincoln Drive, Bethesda, MD, 20852, USA; ; Tel: +1 (301) 827 1914.
出版信息
Soft Matter. 2008;4(4):833-839. doi: 10.1039/b714470a. Epub 2008 Feb 22.
Robust trace-level detection of viruses is crucial to meet urgent needs in fighting the spread of disease or detecting bioterrorism events. We report a new method for rapid and highly sensitive detection of viruses utilizing fluorescent antibody nanotubes. When viral pathogens were mixed with these antibody nanotubes, the nanotubes rapidly aggregated around the viruses to form a networking structure. Trace quantities of viruses such as herpes simplex virus type 2, adenovirus, vaccinia and influenza type B were detected on attomolar order by changes in fluorescence and light scattering intensities associated with aggregation of dye-loaded antibody nanotubes around viruses. High specificity of each antibody nanotube toward its targeted virus was demonstrated by quantifying concentrations of two different viruses in mixtures. This antibody nanotube assay detects targeted pathogens within 30 minutes after incubation with antibody nanotubes. This antibody nanotube assay could fill a pressing need to detect and quantify viruses both rapidly and sensitively.
对病毒进行可靠的痕量检测对于满足抗击疾病传播或检测生物恐怖主义事件的迫切需求至关重要。我们报告了一种利用荧光抗体纳米管快速、高灵敏度检测病毒的新方法。当病毒病原体与这些抗体纳米管混合时，纳米管会迅速聚集在病毒周围形成网络结构。通过与负载染料的抗体纳米管在病毒周围聚集相关的荧光和光散射强度变化，检测到了痕量的病毒，如2型单纯疱疹病毒、腺病毒、痘苗病毒和B型流感病毒，检测限达到阿托摩尔级别。通过对混合物中两种不同病毒的浓度进行定量，证明了每种抗体纳米管对其靶向病毒具有高特异性。这种抗体纳米管检测法在与抗体纳米管孵育后30分钟内即可检测到靶向病原体。这种抗体纳米管检测法可以满足快速、灵敏地检测和定量病毒的迫切需求。
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