Rabalais G P, Stout G G, Ladd K L, Cost K M
Department of Pediatrics, University of Louisville School of Medicine, Kentucky.
J Clin Microbiol. 1992 Jun;30(6):1505-8. doi: 10.1128/jcm.30.6.1505-1508.1992.
We compared the detection of seven respiratory viruses by using a commercially available monoclonal antibody pool in a 2-day shell vial assay with that by using standard cell culture with respiratory syncytial virus (RSV) enzyme-linked immunosorbent assay (ELISA)-negative nasal secretions from hospitalized children. We found 179 respiratory virus isolates by either method in 675 specimens. Overall, the shell vial assay detected 147 of 179 (79%) of the positives after 2 days; cell culture detected 148 of 179 (80%) after a mean incubation period of 7.6 days (range, 1 to 14 days). The sensitivity of the shell vial assay was 78% for RSV, 94% for influenza B virus, 83% for adenovirus, and 80% for parainfluenza viruses. The sensitivity of the cell culture was 70% for RSV, 79% for influenza B virus, 90% for adenovirus, and 89% for parainfluenza viruses. The 2-day shell vial assay allowed the detection of respiratory viruses in a clinically relevant time frame and rapidly detected RSV in specimens lacking RSV antigen by ELISA.
我们将在2天的空斑试验中使用市售单克隆抗体库检测7种呼吸道病毒的情况，与使用标准细胞培养并结合呼吸道合胞病毒（RSV）酶联免疫吸附测定（ELISA）检测住院儿童RSV抗原阴性鼻分泌物的情况进行了比较。我们通过这两种方法在675份标本中发现了179株呼吸道病毒分离株。总体而言，空斑试验在2天后检测出179份阳性样本中的147份（79%）；细胞培养在平均7.6天（范围为1至14天）的孵育期后检测出179份中的148份（80%）。空斑试验对RSV的敏感性为78%，对乙型流感病毒为94%，对腺病毒为83%，对副流感病毒为80%。细胞培养对RSV的敏感性为70%，对乙型流感病毒为79%，对腺病毒为90%，对副流感病毒为89%。2天的空斑试验能够在临床相关的时间范围内检测呼吸道病毒，并能快速检测出ELISA检测缺乏RSV抗原的标本中的RSV。
