Wark Alastair W, Lee Jaeyoung, Kim Suhee, Faisal Shaikh Nayeem, Lee Hye Jin
Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow G1 1XL, UK.
Electrochemical Reaction and Technology Laboratory, Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Republic of Korea.
J Ind Eng Chem. 2010 Mar 25;16(2):169-177. doi: 10.1016/j.jiec.2010.01.061. Epub 2010 Feb 19.
The demand for improved technologies capable of rapidly detecting pathogens with high sensitivity and selectivity in complex environments continues to be a significant challenge that helps drive the development of new analytical techniques. Surface-based detection platforms are particularly attractive as multiple bioaffinity interactions between different targets and corresponding probe molecules can be monitored simultaneously in a single measurement. Furthermore, the possibilities for developing new signal transduction mechanisms alongside novel signal amplification strategies are much more varied. In this article, we describe some of the latest advances in the use of surface bioaffinity detection of pathogens. Three major sections will be discussed: (i) a brief overview on the choice of probe molecules such as antibodies, proteins and aptamers specific to pathogens and surface attachment chemistries to immobilize those probes onto various substrates, (ii) highlighting examples among the current generation of surface biosensors, and (iii) exploring emerging technologies that are highly promising and likely to form the basis of the next generation of pathogenic sensors.
在复杂环境中能够快速、高灵敏度和高选择性地检测病原体的先进技术的需求，仍然是推动新分析技术发展的一项重大挑战。基于表面的检测平台特别有吸引力，因为在单次测量中可以同时监测不同靶标与相应探针分子之间的多种生物亲和相互作用。此外，开发新的信号转导机制以及新型信号放大策略的可能性要丰富得多。在本文中，我们描述了病原体表面生物亲和检测应用方面的一些最新进展。将讨论三个主要部分：（i）简要概述用于病原体的探针分子（如抗体、蛋白质和适体）的选择，以及将这些探针固定到各种基质上的表面附着化学方法；（ii）重点介绍当前一代表面生物传感器中的示例；（iii）探索极具前景且可能成为下一代病原体传感器基础的新兴技术。
