a Department of Oral and Maxillofacial Surgery , Affiliated Stomatological Hospital of Nanchang University, The Key Laboratory of Oral Biomedicine Jiangxi Province, Medical College of Nanchang University , Nanchang , China.
b Comprehensive Breast Health Centre, RuiJin Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):290-299. doi: 10.1080/21691401.2018.1553784.
A variety of surface modification methods are applied to modify titanium implants to improve their biological activity. Micro-arc oxidation (MAO) can relatively simply and efficiently produce porous coatings with high bioactivity and bond strength on titanium surfaces. However, there is no conclusion about the effect of coatings with different pore sizes produced by MAO on bone marrow mesenchymal stem cells (BMSCs). To study the effect of different pore sizes on BMSCs, rat BMSCs were applied to detect the effect of different pore sizes prepared by MAO on cell adhesion and osteogenic differentiation. Three groups of coatings with different pore sizes were successfully prepared, and the pore size was within the range of 3-10 µm. Importantly, the expression of adhesion-related protein integrin β1 and osteogenic-related proteins OSX and ALP increased along with the increase in pore size. This study showed that the porous coating prepared by MAO promotes BMSCs adhesion and osteogenic differentiation. It reveals that the pore size is in the range of 3-10 µm and the larger pores are more beneficial for BMSCs adhesion and osteogenic differentiation. This study is instructive for optimizing the design of biomedical implant surfaces.
各种表面改性方法被应用于修饰钛植入物以提高其生物活性。微弧氧化（MAO）可以相对简单且高效地在钛表面生成具有高生物活性和结合强度的多孔涂层。然而，对于 MAO 生成的不同孔径的涂层对骨髓间充质干细胞（BMSCs）的影响，还没有定论。为了研究不同孔径对 BMSCs 的影响，本研究应用大鼠 BMSCs 来检测 MAO 制备的不同孔径对细胞黏附及成骨分化的影响。成功制备了三组具有不同孔径的涂层，孔径范围在 3-10µm 之间。重要的是，黏附相关蛋白整合素β1和成骨相关蛋白 OSX、ALP 的表达随孔径的增加而增加。本研究表明，MAO 制备的多孔涂层促进了 BMSCs 的黏附和成骨分化。研究揭示了孔径范围在 3-10µm 之间，较大的孔径更有利于 BMSCs 的黏附和成骨分化。该研究对优化生物医学植入物表面的设计具有指导意义。
