Department of Prosthodontics, School and Hospital of Stomatology, Tongji University and Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, PR China.
State Key Laboratory of Oral Diseases, Department of Oral Implant, West China School of Stomatology, Sichuan University, Chengdu, 610041, PR China.
Biochem Biophys Res Commun. 2020 Oct 20;531(3):290-296. doi: 10.1016/j.bbrc.2020.06.158. Epub 2020 Aug 14.
Dental implant is the most effective way to repair the defect or absence of dentition. Currently, the modification in titanium surface properties has become a hot topic in the study of oral implantology. However, more suitable titanium surface coating still needs to be further explored. We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method. We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay. Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection. and the osteogenesis differentiation and neuronal differentiation-related genes were determined by RT-qPCR assay. The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF. Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs. In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
种植牙是修复牙列缺损或缺失的最有效方法。目前，钛表面性能的改良已成为口腔种植学研究的热点。然而，更合适的钛表面涂层仍需要进一步探索。我们通过改进的仿生方法制备了神经生长因子-硫酸软骨素/羟基磷灰石（NGF-CS/HA）-涂层复合钛。我们还通过扫描电子显微镜观察了 NGF-CS/HA 涂层复合钛的表面形貌、厚度、表面附着力和成分分析，并通过 ELISA 鉴定了 NGF 的释放。此外，通过茜素红染色、油红 O 染色和荧光检测对骨髓间充质干细胞（BMSCs）进行鉴定，并通过 RT-qPCR 检测确定成骨分化和神经元分化相关基因。NGF-CS/HA 涂层的厚度为 65.4±6.4μm，表面呈现多孔网络状，NGF-CS/HA 涂层的主要成分是 Ti 和 HA，并且保持了 NGF 的活性和释放。此外，我们成功地获得并鉴定了 BMSCs，并证明 NGF-CS/HA 涂层复合钛能显著上调 BMSCs 中成骨分化和神经元分化相关基因和蛋白的表达水平。综上所述，NGF-CS/HA 涂层复合钛对 BMSCs 向成骨细胞和神经细胞分化具有显著的促进作用。
