Fazekas Reka, Molnár Eszter, Mikecs Barbara, Lohinai Zsolt, Vág János
Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University;
Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University.
J Vis Exp. 2019 Jan 12(143). doi: 10.3791/58535.
Laser speckle contrast imaging (LSCI) is a novel method for measuring superficial blood perfusion over large areas. Since it is non-invasive and avoids direct contact with the measured area, it is suitable for monitoring blood flow changes during wound healing in human patients. Vestibuloplasty is periodontal surgery to the oral vestibule, aiming to restore vestibular depth with simultaneous enlargement of the keratinized gingiva. In this special clinical case, a split thickness flap was elevated at the first upper premolar and a xenogenic collagen matrix was adapted to the resulting recipient bed. LSCI was used to monitor the re- and neovascularization of the graft and the surrounding mucosa for one year. A protocol is introduced for the correct adjustment of microcirculation measurement in the oral mucosa, highlighting difficulties and possible failures. The clinical case study presented demonstrated that - following the appropriate protocol - LSCI is a suitable and reliable method for following up microcirculation in a healing wound in the human oral mucosa and gives useful information on graft integration.
激光散斑对比成像（LSCI）是一种用于大面积测量浅表血液灌注的新方法。由于它是非侵入性的，且避免与测量区域直接接触，因此适用于监测人类患者伤口愈合过程中的血流变化。前庭成形术是针对口腔前庭的牙周手术，旨在恢复前庭深度并同时扩大角化龈。在这个特殊的临床病例中，在上颌第一前磨牙处掀起了一个断层皮瓣，并将异种胶原基质应用于由此产生的受区床。使用LSCI对移植物和周围黏膜的再血管化和新生血管化进行了为期一年的监测。本文介绍了一种在口腔黏膜中正确调整微循环测量的方案，强调了困难和可能出现的失败情况。所呈现的临床病例研究表明，遵循适当的方案，LSCI是一种适用于且可靠的方法，可用于跟踪人类口腔黏膜愈合伤口中的微循环，并提供有关移植物整合的有用信息。
