Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P. R. China.
Biomater Sci. 2019 Mar 26;7(4):1565-1573. doi: 10.1039/c8bm01498a.
Trace rare earth elements such as lanthanum (La) regulated effectively bone tissue performances; however, the underlying mechanism remains unknown. In order to accelerate bone defects especially in patients with osteoporosis or other metabolic diseases, we firstly constructed lanthanum-doped mesoporous calcium silicate/chitosan (La-MCS/CTS) scaffolds by freeze-drying technology. During the freeze-drying procedure, three-dimensional macropores were produced within the La-MCS/CTS scaffolds by using ice crystals as templates, and the La-MCS nanoparticles were distributed on the macropore walls. The hierarchically porous structures and biocompatible components contributed to the adhesion, spreading and proliferation of rat bone marrow-derived mesenchymal stem cells (rBMSCs), and accelerated the in-growth of new bone tissues. Particularly, the La3+ ions in the bone scaffolds remarkably induced the osteogenic differentiation of rBMSCs via the activation of the TGF signal pathway. A critical-sized calvarial-defect rat model further revealed that the La-MCS/CTS scaffolds significantly promoted new bone regeneration as compared with pure MCS/CTS scaffolds. In conclusion, the La-MCS/CTS scaffold showed the prominent ability in osteogenesis and bone regeneration, which showed its application potential for bone defect therapy.
追踪稀土元素（如镧）可以有效调节骨组织性能；然而，其潜在机制尚不清楚。为了加速骨缺损的修复，特别是在骨质疏松症或其他代谢性疾病患者中，我们首先通过冷冻干燥技术构建了镧掺杂介孔硅酸钙/壳聚糖（La-MCS/CTS）支架。在冷冻干燥过程中，通过冰晶作为模板在 La-MCS/CTS 支架内产生了三维大孔，La-MCS 纳米颗粒分布在大孔壁上。分级多孔结构和生物相容性成分有助于大鼠骨髓间充质干细胞（rBMSCs）的黏附、铺展和增殖，并加速新骨组织的生长。特别是，骨支架中的 La3+离子通过激活 TGF 信号通路显著促进了 rBMSCs 的成骨分化。颅骨缺损大鼠模型进一步表明，与纯 MCS/CTS 支架相比，La-MCS/CTS 支架显著促进了新骨的再生。总之，La-MCS/CTS 支架在成骨和骨再生方面表现出突出的能力，显示出其在骨缺损治疗中的应用潜力。
