Blinova Anastasiya A, Karamirzoev Abdurasul A, Guseynova Asiyat R, Maglakelidze David G, Ilyaeva Tatiana A, Gusov Batradz A, Meliksetyants Avetis P, Pirumian Mari M, Taravanov Maxim A, Pirogov Maxim A, Vakalov Dmitriy S, Bernyukevich Tatiana V, Gvozdenko Alexey A, Nagdalian Andrey A, Blinov Andrey V
Department of Physics and Technology of Nanostructures and Materials, Physical and Technical Faculty, North Caucasus Federal University, 355017 Stavropol, Russia.
Faculty of Dentistry, North Ossetian State Medical University, 362025 Vladikavkaz, Russia.
Micromachines (Basel). 2023 Jan 18;14(2):245. doi: 10.3390/mi14020245.
This work presents the development of a method for the synthesis of calcium silicate nanoparticles stabilized with essential amino acids. CaSiO nanoparticles were obtained through chemical precipitation. In the first stage, the optimal calcium-containing precursor was determined. The samples were examined using scanning electron microscopy. It was found that Ca(CHCOO) was the optimal calcium-containing precursor. Then, the phase composition of calcium silicate was studied using X-ray phase analysis. The results showed the presence of high-intensity bands in the diffractogram, which characterized the phase of the nanosized CaSiO-wollastonite. In the next stage, the influence of the type of amino acid on the microstructure of calcium silicate was studied. The amnio acids studied were valine, L-leucine, L-isoleucine, L-methionine, L-threonine, L-lysine, L-phenylalanine, and L-tryptophan. The analysis of the SEM micrographs showed that the addition of amino acids did not significantly affect the morphology of the CaSiO samples. The surface of the CaSiO samples, both without a stabilizer and with amino acids, was represented by irregularly shaped aggregates consisting of nanoparticles with a diameter of 50-400 nm. Further, in order to determine the optimal amino acid to use to stabilize nanoparticles, computerized quantum chemical modeling was carried out. Analysis of the data obtained showed that the most energetically favorable interaction was the CaSiO-L-methionine configuration, where the interaction occurs through the amino group of the amino acid; the energy value of which was -2058.497 kcal/mol. To confirm the simulation results, the samples were examined using IR spectroscopy. An analysis of the results showed that the interaction of calcium silicate with L-methionine occurs via the formation of a bond through the NH group of the amino acid.
这项工作展示了一种用必需氨基酸稳定的硅酸钙纳米颗粒的合成方法的开发。通过化学沉淀法获得了CaSiO纳米颗粒。在第一阶段，确定了最佳的含钙前驱体。使用扫描电子显微镜对样品进行了检查。发现Ca(CHCOO)是最佳的含钙前驱体。然后，使用X射线相分析研究了硅酸钙的相组成。结果表明，衍射图中存在高强度谱带，这表征了纳米级CaSiO-硅灰石的相。在下一阶段，研究了氨基酸类型对硅酸钙微观结构的影响。所研究的氨基酸有缬氨酸、L-亮氨酸、L-异亮氨酸、L-蛋氨酸、L-苏氨酸、L-赖氨酸、L-苯丙氨酸和L-色氨酸。扫描电子显微镜图像分析表明，添加氨基酸对CaSiO样品的形态没有显著影响。无论有无稳定剂，CaSiO样品的表面均由直径为50 - 400 nm的纳米颗粒组成的不规则形状聚集体构成。此外，为了确定用于稳定纳米颗粒的最佳氨基酸，进行了计算机化量子化学建模。对所得数据的分析表明，能量上最有利的相互作用是CaSiO-L-蛋氨酸构型，其中相互作用通过氨基酸的氨基发生；其能量值为-2058.497 kcal/mol。为了证实模拟结果，使用红外光谱对样品进行了检查。结果分析表明，硅酸钙与L-蛋氨酸的相互作用是通过氨基酸的NH基团形成键而发生的。
