Bayram Cem, Ozturk Sukru, Karaosmanoglu Beren, Gultekinoglu Merve, Taskiran Ekim Z, Ulubayram Kezban, Majd Hamta, Ahmed Jubair, Edirisinghe Mohan
Department of Nanotechnology and Nanomedicine, Graduate School of Science and Engineering, Hacettepe University, Beytepe, Ankara, 06800, Turkey.
Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Hacettepe University, Ankara, 06100, Turkey.
Macromol Biosci. 2024 Dec;24(12):e2400279. doi: 10.1002/mabi.202400279. Epub 2024 Oct 10.
The combination of gelatin and hydroxyapatite (HA) has emerged as a promising strategy in dental tissue engineering due to its favorable biocompatibility, mechanical properties, and ability to support cellular activities essential for tissue regeneration, rendering them ideal components for hard tissue applications. Besides, precise control over interconnecting porosity is of paramount importance for tissue engineering materials. Conventional methods for creating porous scaffolds frequently encounter difficulties in regulating pore size distribution. This study demonstrates the fabrication of gelatin-nano HA scaffolds with uniform porosity using a T-type junction microfluidic device in a single-step process. Significant improvements in control over the pore size distribution are achieved by regulating the flow parameters, resulting in effective and time-efficient manufacturing comparable in quality to the innovative 3D bioprinting techniques. The overall porosity of the scaffolds exceeded 60%, with a remarkably narrow size distribution. The incorporation of nano-HAinto 3D porous gelatin scaffolds successfully induced osteogenic differentiation in stem cells at both the protein and gene levels, as evidenced by the significant increase in osteocalcin (OCN), an important marker of osteogenic differentiation. The OCN levels are 26 and 43 times higher for gelatin and gelatin-HA scaffolds, respectively, compared to the control group.
由于明胶和羟基磷灰石（HA）具有良好的生物相容性、机械性能以及支持组织再生所必需的细胞活动的能力，它们的组合已成为牙科组织工程中一种很有前景的策略，使其成为硬组织应用的理想成分。此外，精确控制相互连通的孔隙率对于组织工程材料至关重要。传统的制备多孔支架的方法在调节孔径分布方面经常遇到困难。本研究展示了使用T型连接微流控装置通过一步法制备具有均匀孔隙率的明胶-纳米HA支架。通过调节流动参数，在孔径分布的控制方面取得了显著改进，从而实现了高效且省时的制造，其质量可与创新的3D生物打印技术相媲美。支架的总孔隙率超过60%，尺寸分布非常狭窄。将纳米HA掺入3D多孔明胶支架中，在蛋白质和基因水平上均成功诱导了干细胞的成骨分化，成骨分化的重要标志物骨钙素（OCN）显著增加证明了这一点。与对照组相比，明胶支架和明胶-HA支架的OCN水平分别高出26倍和43倍。
