Afouda Pamela, Traore Sory Ibrahima, Dione Niokhor, Andrieu Claudia, Tomei Enora, Richez Magali, Di Pinto Fabrizio, Lagier Jean-Christophe, Dubourg Grégory, Raoult Didier, Fournier Pierre-Edouard
IRD, AP-HM, MEPHI, IHU-Méditerranée Infection, Aix Marseille University, Marseille, France.
Canada Excellence Research Chair Microbiome-Endocannabinoidome Axis in Metabolic Health (CERC-MEND), Université Laval, Quebec City, Canada.
Antonie Van Leeuwenhoek. 2019 Jun;112(6):905-918. doi: 10.1007/s10482-018-01223-x. Epub 2019 Jan 28.
Using the culturomics approach, we isolated two strains, Marseille-P2963 and Marseille-P3753, from the intestinal microbiota of a 19-year-old healthy Saudi Arabian Bedouin male and from a 32-year-old healthy Senegalese male faecal transplant donor. Here, we studied their phenotypic, phylogenetic and genomic characteristics. Both strains were phylogenetically related, but different from Ruminococcus species. Bacterial cells were anaerobic, rod-shaped, non-spore-forming and not motile, with neither catalase nor oxidase activities. Their growth temperatures ranged from 28 to 45 °C, with an optimal growth at 37 °C. The genomes are 2,842,720 bp- and 2,707,061 bp-long respectively. The G + C contents are 47.18% and 46.90%, respectively. Based on these characteristics, we propose the creation of a new genus within the family Ruminococcaceae named Massiliimalia gen. nov., that contains the new species Massiliimalia massiliensis gen. nov., sp. nov., and Massiliimalia timonensis gen. nov., sp. nov. Strains Marseille-P2963 (= CSUR P2963 = DSM 106837) and Marseille-P3753 (= CSUR P3753 = CCUG 71632) are their type strains, respectively.
采用文化组学方法，我们从一名19岁健康沙特阿拉伯贝都因男性的肠道微生物群以及一名32岁健康塞内加尔男性粪便移植供体中分离出两株菌株，分别为马赛 - P2963和马赛 - P3753。在此，我们研究了它们的表型、系统发育和基因组特征。这两株菌株在系统发育上相关，但与瘤胃球菌属不同。细菌细胞为厌氧、杆状、不形成芽孢且无运动性，既无过氧化氢酶活性也无氧化酶活性。它们的生长温度范围为28至45°C，最适生长温度为37°C。基因组长度分别为2,842,720 bp和2,707,061 bp。G + C含量分别为47.18%和46.90%。基于这些特征，我们提议在瘤胃球菌科内创建一个新属，命名为马西利马利亚属（Massiliimalia gen. nov.），该属包含新物种马西利马利亚马西利ensis（Massiliimalia massiliensis gen. nov., sp. nov.）和马西利马利亚蒂蒙ensis（Massiliimalia timonensis gen. nov., sp. nov.）。菌株马赛 - P2963（= CSUR P2963 = DSM 106837）和马赛 - P3753（= CSUR P3753 = CCUG 71632）分别为它们的模式菌株。
