Research Center for Bio-Based Chemistry, Korea Research Institute of Chemical Technology (KRICT) , University of Science and Technology (UST) , Ulsan 44429 , Korea.
Department of Nanomechanics, Korea Institute of Machinery and Materials (KIMM) , University of Science and Technology (UST) , 156 Gajeongbuk-ro , Yuseong-gu, Daejeon 34103 , Korea.
ACS Appl Mater Interfaces. 2019 Mar 13;11(10):9786-9793. doi: 10.1021/acsami.8b21569. Epub 2019 Feb 7.
Although biodegradable membranes are essential for effective bone repair, severe loss of mechanical stability because of rapid biodegradation, soft tissue invasion, and excessive immune response remain intrinsically problematic. Inspired by the exoskeleton-reinforcing strategy found in nature, we have produced a Ti-infiltrated chitin nanofibrous membrane. The membrane employs vapor-phase infiltration of metals, which often occurs during metal oxide atomic layer deposition (ALD) on organic substrates. This metal infiltration manifests anomalous mechanical improvement and stable integration with chitin without cytotoxicity and immunogenicity. The membrane exhibits both impressive toughness (∼13.3 MJ·m) and high tensile strength (∼55.6 MPa), properties that are often mutually exclusive. More importantly, the membrane demonstrates notably enhanced resistance to biodegradation, remaining intact over the course of 12 weeks. It exhibits excellent osteointegrative performance and suppresses the immune response to pathogen-associated molecular pattern molecules indicated by IL-1β, IL-6, and granulocyte-macrophage colony-stimulating factor expression. We believe the excellent chemico-biological properties achieved with ALD treatment can provide insight for synergistic utilization of the polymers and ALD in medical applications.
虽然可生物降解膜对于有效的骨修复至关重要，但由于快速的生物降解、软组织入侵和过度的免疫反应，其机械稳定性严重丧失仍然是一个内在问题。受自然界中发现的外骨骼增强策略的启发，我们制备了一种钛渗透甲壳素纳米纤维膜。该膜采用气相金属渗透法，这种方法通常发生在金属氧化物原子层沉积（ALD）在有机基底上的时候。这种金属渗透表现出异常的机械增强和与甲壳素的稳定整合，同时没有细胞毒性和免疫原性。该膜具有令人印象深刻的韧性（约 13.3MJ·m）和高拉伸强度（约 55.6MPa），这些性能通常是相互排斥的。更重要的是，该膜对生物降解的抵抗力明显增强，在 12 周的时间内保持完整。它表现出优异的骨整合性能，并抑制了与病原体相关的分子模式分子的免疫反应，这些分子的表达通过 IL-1β、IL-6 和粒细胞-巨噬细胞集落刺激因子的表达来指示。我们相信，通过 ALD 处理实现的优异的化学-生物学性能可以为聚合物和 ALD 在医学应用中的协同利用提供启示。
