College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, PR China.
Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, PR China.
Biosens Bioelectron. 2019 Mar 15;129:132-138. doi: 10.1016/j.bios.2019.01.005. Epub 2019 Jan 11.
The redox balance in cellular mitochondria is closely related to the physiological and pathological processes of the body. When exposed to external stimuli, the redox state in cells changes dynamically, and presents cell heterogeneity, which creates a need for techniques that can make dynamic and reversible visual analysis of redox in mitochondria at single-cell level. Here we describe a method for single-cell redox analysis based on a microfluidic device combing with a reversible fluorescent probe (Cy-O-ebselen), that enables online culture, labelling and dynamic fluorescent imaging analysis of mitochondrial redox (HO/GSH) change. Using this method, we further explored the dynamic changes of mitochondrial redox state after thermal stimulation or combined thermal-drug stimulation, and analysed the heterogeneous response of cells to external stimuli at the single cell level.
细胞线粒体中的氧化还原平衡与身体的生理和病理过程密切相关。当暴露于外部刺激时，细胞中的氧化还原状态会动态变化，并呈现出细胞异质性，这就需要能够在单细胞水平上对线粒体中的氧化还原进行动态和可逆荧光分析的技术。在这里，我们描述了一种基于微流控装置结合可逆荧光探针（Cy-O-ebselen）的单细胞氧化还原分析方法，该方法能够在线培养、标记和动态荧光成像分析线粒体氧化还原（HO/GSH）变化。使用该方法，我们进一步探讨了热刺激或联合热药物刺激后线粒体氧化还原状态的动态变化，并在单细胞水平上分析了细胞对外界刺激的异质反应。
