比较左侧或右侧迷走神经切断术在急性胰腺炎大鼠中的作用。

Comparison of left side or right side vagotomy in the rat subjected to acute pancreatitis.

机构信息

Department of Medical Physiology, Faculty of Health Sciences, Jagiellonian University Medical College, Krakow, Poland.

Department of Physiology, Medical Faculty, Jagiellonian University Medical College, Krakow, Poland.

出版信息

Adv Med Sci. 2019 Mar;64(1):162-168. doi: 10.1016/j.advms.2018.10.001. Epub 2019 Jan 25.

DOI:10.1016/j.advms.2018.10.001

PMID:30690339

Abstract

PURPOSE

We aimed to evaluate the effects of unilateral vagotomy (right-VR or left-VL) on the severity of caerulein-induced acute pancreatitis (AP).

MATERIAL AND METHODS

VR or VL was done in Wistar rats 4 days before AP, except in control, sham operated group. Following 5 h administration of subcutaneous injections of caerulein, the pancreatic blood flow (PBF), serum lipase and IL-10 in caval blood samples were measured. The pancreatic specimens were taken from sacrificed rats for the assessment of MDA-4-HNE and morphology.

RESULTS

PBF decreased from 310 ± 20 ml/min/100 g of tissue in control rats to 130 ± 12 units in AP (p < 0.01). VR and VL alleviated this effect to 234 ± 22 and 229 ± 26 units, respectively, (p < 0.01). There was an immense increase of serum lipase in AP, from 100 ± 7 U/L up to 5220 ± 210 U/L (p < 0.01). Only VL limited this increase to 3469 ± 300 U/L (p < 0.01). Serum IL-10 increased uniformly in AP, without any effect of preceding VR or VL. VL performed in rats subjected subsequently to AP resulted in stronger reduction of histological changes, such as pancreatic edema and leukocyte infiltration, than the above parameters in AP rats with VR. MDA+4-HNE increased from 7.5 ± 0.1 pmol/g of tissue in control group to 30.6 ± 3 units in AP group (p < 0.01). Concentration of MDA+4-HNE in pancreatic tissue achieved 16.48 ± 3 pmol/g after VR and 13.84 ± 4 pmol/g following VL.

CONCLUSION

Our observation might suggest that protective effect of VL could be stronger than VR in the protection on AP. However changes of PBF seem to be similar in both groups of rats.

摘要

目的

本研究旨在评估单侧迷走神经切断术（右侧-VR 或左侧-VL）对蛙皮素诱导的急性胰腺炎（AP）严重程度的影响。

材料与方法

AP 前 4 天，在 Wistar 大鼠中进行 VR 或 VL，除对照组和假手术组外。在皮下注射蛙皮素 5 小时后，测量腔静脉血样中的胰腺血流（PBF）、血清脂肪酶和白细胞介素-10。从处死的大鼠中取出胰腺标本，评估 MDA-4-HNE 和形态学。

结果

与对照组大鼠的 310±20ml/min/100g 组织相比，AP 大鼠的 PBF 下降至 130±12 单位（p<0.01）。VR 和 VL 分别减轻至 234±22 和 229±26 单位（p<0.01）。AP 大鼠血清脂肪酶大量增加，从 100±7U/L 增加至 5220±210U/L（p<0.01）。只有 VL 将这种增加限制在 3469±300U/L（p<0.01）。AP 大鼠血清白细胞介素-10 均匀增加，而 VR 对其无影响。AP 后进行 VL 的大鼠的组织学变化（如胰腺水肿和白细胞浸润）的减轻程度强于 AP 大鼠的上述参数。与对照组的 7.5±0.1pmol/g 组织相比，AP 组的 MDA+4-HNE 增加到 30.6±3 单位（p<0.01）。VR 后胰腺组织中 MDA+4-HNE 浓度达到 16.48±3pmol/g，VL 后达到 13.84±4pmol/g。