Department of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, School of Medicine, University of Catania, Via S. Sofia n°87, 95124 Catania, Italy.
School of Life Sciences, Faculty of Science, University of Technology Sydney, P.O. Box 123, Broadway, Sydney, NSW 2007, Australia.
Int J Mol Sci. 2019 Jan 25;20(3):511. doi: 10.3390/ijms20030511.
The purpose of this study was to investigate the influence of moderate physical activity (MPA) on the expression of osteoarthritis (OA)-related (IL-1β, IL-6, TNF-α, MMP-13) and anti-inflammatory and chondroprotective (IL-4, IL-10, lubricin) biomarkers in the synovium of an OA-induced rat model. A total of 32 rats were divided into four groups: Control rats (Group 1); rats performing MPA (Group 2); anterior cruciate ligament transection (ACLT)-rats with OA (Group 3); and, ACLT-rats performing MPA (Group 4). Analyses were performed using Hematoxylin & Eosin (H & E) staining, histomorphometry and immunohistochemistry. In Group 3, OA biomarkers were significantly increased, whereas, IL-4, IL-10, and lubricin were significantly lower than in the other experimental groups. We hypothesize that MPA might partake in rescuing type B synoviocyte dysfunction at the early stages of OA, delaying the progression of the disease.
本研究旨在探讨适度体力活动（MPA）对骨关节炎（OA）相关（IL-1β、IL-6、TNF-α、MMP-13）和抗炎及软骨保护（IL-4、IL-10、润滑素）生物标志物在 OA 诱导的大鼠模型滑膜中表达的影响。共 32 只大鼠分为四组：对照组大鼠（第 1 组）；进行 MPA 的大鼠（第 2 组）；前交叉韧带切断（ACLT）-OA 大鼠（第 3 组）；以及进行 MPA 的 ACLT 大鼠（第 4 组）。使用苏木精和伊红（H & E）染色、组织形态计量学和免疫组织化学进行分析。在第 3 组中，OA 生物标志物显著增加，而 IL-4、IL-10 和润滑素显著低于其他实验组。我们假设 MPA 可能参与在 OA 早期挽救 B 型滑膜细胞功能障碍，从而延缓疾病的进展。
