Institute for Laboratory Animal Science and Experimental Surgery, University Hospital RWTH Aachen, Aachen, Germany; HPB Research Center Hungary, 1st Department of Surgery, Semmelweis University, Budapest, Hungary; Doctoral School of Clinical Medicine, Semmelweis University, Budapest, Hungary.
HPB Research Center Hungary, 1st Department of Surgery, Semmelweis University, Budapest, Hungary.
J Surg Res. 2019 Mar;235:578-590. doi: 10.1016/j.jss.2018.10.038. Epub 2018 Dec 5.
Portal vein occlusion and associating liver partition and portal vein ligation for staged hepatectomy techniques are in the spotlight of oncological liver surgery. Research involving animal models is indispensable to study the mechanisms of liver regeneration. Inaccurate reporting acts as a significant barrier during the correct interpretation of preclinical findings. Hence, we performed a systematic review to evaluate the status quo of the reporting standards and to assess the potential factors influencing reporting in animal studies, which are focusing on portal vein occlusion and/or associating liver partition and portal vein ligation for staged hepatectomy techniques.
A systematic review was performed in the PubMed and Ovid MEDLINE databases. Baseline study characteristics were recorded, and quality assessment was performed using the Animals in Research: Reporting in vivo Experiments (ARRIVE) checklist.
A total of 107 research articles were included for the comprehensive assessment. In the subgroup analysis, newer reports and studies from the post-ARRIVE era, and reports from Europe were all associated with significantly higher ARRIVE scores (P < 0.05). Univariable regression analysis confirmed these factors as predictors of higher reporting quality. However, in the multivariable analysis, only publishing in the post-ARRIVE era has been found as single independent predictor of higher reporting standards (P = 0.028 post-ARRIVE total score 75th percentile; P = 0.000 post-ARRIVE total score median).
Although an improving trend has been observed in reporting quality over the past years, this effect was clearly insufficient. Our results emphasize the need for further measures to improve the methodical quality at all levels of planning, execution, and reporting of preclinical studies in liver regeneration research.
门静脉阻断联合肝脏离断和门静脉结扎的分阶段肝切除术技术是肿瘤肝脏手术的焦点。研究动物模型对于研究肝脏再生的机制是必不可少的。不准确的报告是正确解释临床前发现的一个重大障碍。因此，我们进行了系统评价，以评估报告标准的现状，并评估影响聚焦于门静脉阻断和/或联合肝脏离断和门静脉结扎的分阶段肝切除术技术的动物研究报告的潜在因素。
在 PubMed 和 Ovid MEDLINE 数据库中进行了系统评价。记录了基线研究特征，并使用动物在研究中的报告：体内实验 (ARRIVE) 检查表进行了质量评估。
共纳入 107 篇研究文章进行综合评估。在亚组分析中，更新的报告和 ARRIVE 时代后的研究，以及来自欧洲的报告，ARRIVE 评分均显著较高（P < 0.05）。单变量回归分析证实了这些因素是报告质量较高的预测因素。然而，在多变量分析中，只有在 ARRIVE 时代后发表被发现是较高报告标准的唯一独立预测因素（ARRIVE 总分第 75 百分位数，P = 0.028；ARRIVE 总分中位数，P = 0.000）。
尽管近年来报告质量有了提高的趋势，但这种效果显然还不够。我们的结果强调需要采取进一步措施，在肝脏再生研究中提高临床前研究在规划、执行和报告各个层面的方法学质量。
