Department of Neuroscience, Baylor College of Medicine, Houston, United States.
Center for Neuroscience and Artificial Intelligence, Baylor College of Medicine, Houston, United States.
Elife. 2020 Mar 5;9:e52951. doi: 10.7554/eLife.52951.
Clones of excitatory neurons derived from a common progenitor have been proposed to serve as elementary information processing modules in the neocortex. To characterize the cell types and circuit diagram of clonally related excitatory neurons, we performed multi-cell patch clamp recordings and Patch-seq on neurons derived from -positive progenitors labeled by tamoxifen induction at embryonic day 10.5. The resulting clones are derived from two radial glia on average, span cortical layers 2-6, and are composed of a random sampling of transcriptomic cell types. We find an interaction between shared lineage and connection type: related neurons are more likely to be connected vertically across cortical layers, but not laterally within the same layer. These findings challenge the view that related neurons show uniformly increased connectivity and suggest that integration of vertical -clonal input with lateral -clonal input may represent a developmentally programmed connectivity motif supporting the emergence of functional circuits.
从共同前体中衍生的兴奋性神经元克隆被提议作为新皮层中基本信息处理模块。为了描述克隆相关兴奋性神经元的细胞类型和电路图，我们对通过在胚胎第 10.5 天用他莫昔芬诱导标记的 -阳性祖细胞衍生的神经元进行了多细胞膜片钳记录和 Patch-seq。所得克隆平均来自两个放射状胶质细胞，跨越皮层 2-6 层，并由转录组细胞类型的随机抽样组成。我们发现共享谱系和连接类型之间存在相互作用：相关神经元更有可能在皮层层之间垂直连接，但不在同一层内横向连接。这些发现挑战了相关神经元表现出均匀增加的连接性的观点，并表明垂直 -克隆输入与侧向 -克隆输入的整合可能代表一种发育编程的连接模式，支持功能电路的出现。
