State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, Jiangsu, PR China.
Sci Rep. 2019 Jan 28;9(1):805. doi: 10.1038/s41598-018-34379-7.
In this study, complex structured soluble lignin wastewater was treated by electro-microbial system (EMS) using different direct current (DC) application modes (CR (continuous ON), IR (12 h-ON/12 h-OFF) and IR (2 h-ON/2 h-OFF)), and physiological characteristics and microbial communities were investigated. Results showed that CR, IR and IR had higher lignin removals, which were almost two times that of the control reactor (R', no current), and IR performed best and stably. Furthermore, IR exhibited the lowest ohmic resistance (Rs) of electrode biofilms, which could be explained by its higher abundance of electroactive bacteria. In the activated sludge of EMS, the concentration of dehydrogenase activity (DHA) and electronic transport system (ETS) in IR were the highest (1.48 and 1.28 times of R'), which contributed to its high content of adenosine triphosphate (ATP). The viability of activated sludge was not affected by different DC application modes. Phospholipid fatty acids (PLFA) analysis indicated that IR had the maximum content of C15:1 anteiso A, C16:0 and C18:0; CR increased the content of C15:0 anteiso and decreased the content of saturated fatty acids. Genus-level results revealed that lignin-degrading bacteria, Pseudoxanthomonas and Mycobacterium, could be enriched in IR and CR, respectively.
在这项研究中，采用不同的直流（DC）应用模式（连续 ON 的 CR、12 h-ON/12 h-OFF 的 IR 和 2 h-ON/2 h-OFF 的 IR）的电微生物系统（EMS）处理复杂结构的可溶性木质素废水，并研究了生理特性和微生物群落。结果表明，CR、IR 和 IR 具有更高的木质素去除率，几乎是对照反应器（无电流，R'）的两倍，IR 表现最好且稳定。此外，IR 表现出电极生物膜的最低欧姆电阻（Rs），这可以通过其更高丰度的电活性细菌来解释。在 EMS 的活性污泥中，IR 中的脱氢酶活性（DHA）和电子传递系统（ETS）浓度最高（分别为 R'的 1.48 和 1.28 倍），这有助于其含有较高浓度的三磷酸腺苷（ATP）。不同 DC 应用模式对活性污泥的存活能力没有影响。磷脂脂肪酸（PLFA）分析表明，IR 具有最大含量的 C15:1 anteiso A、C16:0 和 C18:0；CR 增加了 C15:0 anteiso 的含量并降低了饱和脂肪酸的含量。属水平的结果表明，木质素降解菌 Pseudoxanthomonas 和 Mycobacterium 分别可以在 IR 和 CR 中得到富集。
