State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, Jiangsu, PR China.
Bioresour Technol. 2018 Jan;247:319-326. doi: 10.1016/j.biortech.2017.09.082. Epub 2017 Sep 13.
In order to explore physicochemical and biological effects on detachment and activity recovery of aging biofilm by enzyme and surfactant treatments, two kinds of biofilm processes, i.e. biological aeration filter (BAF) and moving bed biofilm reactor (MBBR), and multiple indicators including water quality, biofilm morphology, activity and microbial community structure, were employed. Results showed that detachment of aging biofilm was mainly attributed by extracellular polymeric substance (EPS) solubilization and dispersion, and activity recovery of aging biofilm mainly depended on biological effects of dominant bacteria. Phosphorus metabolism related bacteria, such as Microbacterium and Micropruina, were responsible for BAF biofilm regeneration. More abundant microbial community structure of MBBR regenerated biofilm was found, and biofilm activity was not only related to phosphorus metabolism related bacteria, but also to denitrifying bacteria. Rhamnolipid performed best on aging biofilm detachment and regeneration, giving a clue for effective activation of aging biofilm in wastewater treatment systems.
为了探索酶和表面活性剂处理对老化生物膜脱落和活性恢复的理化及生物学效应，采用了两种生物膜工艺，即曝气生物滤池（BAF）和移动床生物膜反应器（MBBR），以及包括水质、生物膜形态、活性和微生物群落结构在内的多个指标。结果表明，老化生物膜的脱落主要归因于胞外聚合物（EPS）的溶解和分散，而老化生物膜的活性恢复主要取决于优势细菌的生物效应。磷代谢相关细菌，如 Microbacterium 和 Micropruina，负责 BAF 生物膜的再生。MBBR 再生生物膜的微生物群落结构更加丰富，生物膜活性不仅与磷代谢相关细菌有关，还与反硝化细菌有关。鼠李糖脂对老化生物膜的脱落和再生效果最佳，为废水处理系统中有效激活老化生物膜提供了线索。
