Javidanpour Somayeh, Dianat Mahin, Aliakbari Fatemeh Ramezani, Sarkaki Alireza
Student Research Committee, Science and Religion Work Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Physiology and Persian Gulf Physiology Research Centre, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Res Med Sci. 2018 Dec 28;23:108. doi: 10.4103/jrms.JRMS_517_18. eCollection 2018.
There is evidence that regular activity can prevent of cardiovascular diseases. There are many reports that exercise and the consumption of olive leaf extract (OLE) have a positive effect on cardiovascular parameters. This study was conducted to compare the effects of exercise and OLE alone and together on electrocardiographic parameters in rats.
Male Sprague-Dawley rats were randomly divided into six groups ( = 8 rats in each): Control, exercise, OLE (100, 200, and 400 mg/kg, orally for 14 days), and exercise + OLE (200 mg/kg of extract, orally for 14 days). Exercise training in rats was performed using treadmill for 28 days (1 h/day). Electrophysiological parameters including heart rate, PR interval, QT interval, QT corrected (QTc), RR interval, QRS voltage, and duration were obtained from lead II electrocardiogram (ECG) recorded by a PowerLab system. Statistical evaluation was done by one-way analysis of variance followed by Fisher's least significant difference test and < 0.05 was considered statistically significant.
The amounts of QT ( = 0.0009) and QTc interval ( = 0.0004), RR interval ( < 0.0001), QRS duration ( = 0.004), and QRS voltage ( = 0.003) in the exercise group were significantly higher than those of the control group. However, there were no significant differences in PR interval in comparison with the control group. Exercise ( < 0.0001) and OLE (400 mg/kg, = 0.043) alone and both in combination ( = 0.007) reduced heart rate and increased the amount of QRS voltage ( = 0.003, = 0.047, and = 0.046, respectively) and RR interval ( < 0.0001, = 0.046, and = 0.0009, respectively).
Results of this study indicated that administration of OLE alone and in combination with exercise has negative chronotropic and positive inotropic effects and also it can prevent of prolongation of QT and QTc interval induced by severe exercise.
有证据表明规律运动可预防心血管疾病。有许多报道称运动和食用橄榄叶提取物（OLE）对心血管参数有积极影响。本研究旨在比较单独运动、单独使用OLE以及运动与OLE联合使用对大鼠心电图参数的影响。
将雄性Sprague-Dawley大鼠随机分为六组（每组n = 8只大鼠）：对照组、运动组、OLE组（100、200和400 mg/kg，口服14天）以及运动 + OLE组（200 mg/kg提取物，口服14天）。大鼠的运动训练使用跑步机进行28天（每天1小时）。通过PowerLab系统记录II导联心电图（ECG）获取包括心率、PR间期、QT间期、校正QT（QTc）、RR间期、QRS电压和时限等电生理参数。采用单因素方差分析，随后进行Fisher最小显著差异检验进行统计评估，P < 0.05被认为具有统计学意义。
运动组的QT（P = 0.0009）和QTc间期（P = 0.0004）、RR间期（P < 0.0001）、QRS时限（P = 0.004）以及QRS电压（P = 0.003）均显著高于对照组。然而，与对照组相比，PR间期无显著差异。单独运动（P < 0.0001）、单独使用OLE（400 mg/kg，P = 0.043）以及两者联合使用（P = 0.007）均可降低心率，并增加QRS电压（分别为P = 0.003、P = 0.047和P = 0.046）以及RR间期（分别为P < 0.0001、P = 0.046和P = 0.0009）。
本研究结果表明，单独使用OLE以及与运动联合使用具有负性变时作用和正性变力作用，并且还可预防剧烈运动引起的QT和QTc间期延长。
