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在新型细胞培养装置中长期培养 。

Long-Term Cultivation of in a Novel Cell Culture Device.

机构信息

Centre François Baclesse, Normandie Université, UNICAEN, UR ABTE EA 4651, Caen, France.

Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.

出版信息

Am J Trop Med Hyg. 2019 Apr;100(4):822-827. doi: 10.4269/ajtmh.18-0527.

DOI:10.4269/ajtmh.18-0527
PMID:30693863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6447126/
Abstract

The standard cultivation procedure for requires gas exchange and a microaerophilic atmosphere. A novel system using a commercially available cell culture device (Petaka G3; Celartia Ltd., Powell, OH) was assessed for long-term cultivation of a reference laboratory clone in normal air. Parasite growth during 30 days was similar, or better, in Petaka G3 than that in the standard cultivation method with gas exchange in a CO incubator. The successful cultivation of in the Petaka G3 device suggests that low O content available in hemoglobin and dissolved gas in the blood is sufficient for long-term cultivation. This finding may open the way to novel methods to cultivate and adapt field isolates to conditions with more ease.

摘要

该标准的培养程序为需要气体交换和微需氧环境。一个新颖的系统，使用市售的细胞培养设备（Petaka G3；Celartia 有限公司，鲍威尔，俄亥俄州），评估了长期培养的一个参考实验室克隆在正常空气中。寄生虫生长在 30 天内是相似的，或更好的，在 Petaka G3 比在标准培养方法中的气体交换在一个 CO 培养箱。成功的培养为在 Petaka G3 设备表明，低 O 含量可利用的血红蛋白和溶解气体在血液中是足够的，为长期培养。这一发现可能开辟新的方法来培养和适应现场孤立的条件下更容易。

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