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[利用脐带红细胞进行恶性疟原虫的体外培养]

[In vitro cultivation of Plasmodium falciparum with umbilical cord erythrocyte].

作者信息

Li C M, Su T C

机构信息

Department of Parasitology, Wuhan Medical School for Metallurgical Industry.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1990;8(4):281-3.

PMID:2099258
Abstract

Plasmodium falciparum was cultivated with umbilical cord erythrocytes or with erythrocytes from human adults for 33 days and 50 days respectively. The erythrocyte infection rate increased eightfold to eighteenfold at intervals of three to four days, the highest erythrocyte infection rate being more than 20%. Furthermore, the infection rate of umbilical cord erythrocytes was higher than that of adult erythrocytes at 48, 72 and 96 hours of cultivation, respectively (P less than 0.01). The results suggested that human umbilical cord blood might be a good source of erythrocytes for in vitro cultivation of malaria parasite.

摘要

恶性疟原虫分别用人脐血红细胞或成人红细胞培养33天和50天。红细胞感染率每隔三到四天增加八倍至十八倍,最高红细胞感染率超过20%。此外,在培养48、72和96小时时,脐血红细胞的感染率分别高于成人红细胞(P小于0.01)。结果表明,人脐血可能是体外培养疟原虫的良好红细胞来源。

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