1Department of Genetic Engineering, Biotechnology Centre, Ministry of Environment, Doha, Qatar.
2Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, Key Biscayne, Florida, USA.
Int J Syst Evol Microbiol. 2019 Mar;69(3):839-845. doi: 10.1099/ijsem.0.003250. Epub 2019 Jan 29.
Two strains, 1Y129 and 3Y383, which represent a single novel anamorphic yeast species, were isolated from the Arabian Gulf surrounding Qatar. The two strains have identical sequences in the D1/D2 domains of the large subunit (LSU) and the internal transcribed spacer (ITS) regions of the rRNA gene. On the basis of this sequence analysis the two strains were found to be closely related to Cystobasidium slooffiae CBS5706 with 97. 3 % nucleotide substitutions (12 nt; four gaps) in D1/D2 regions of the LSU rRNA and 97.0 % nucleotide substitutions (14 nt; two gaps) in ITS regions. In contrast to Cystobasidium halotolerans sp. nov., the related species Cystobasidium slooffiae, Cystobasidium minutum and Cystobasidium fimetarium were unable to assimilate d-galactose, soluble starch, galactitol, methanol or nitrate, and grew at 37 °C. The name proposed for this undescribed species is Cystobasidium halotolerans sp. nov. The ex-type strain is QCC/Y31/17 (=MUCL057192=CGMCC2.5570).
两株菌，1Y129 和 3Y383，代表了一种新型的无性酵母种，从卡塔尔周围的阿拉伯海湾中分离得到。这两株菌在大亚基（LSU）的 D1/D2 结构域和 rRNA 基因的内部转录间隔区（ITS）区域具有完全相同的序列。基于这一序列分析，这两株菌与 Cystobasidium slooffiae CBS5706 密切相关，在 LSU rRNA 的 D1/D2 区域有 97.3%的核苷酸替换（12 个核苷酸；4 个空位），在 ITS 区域有 97.0%的核苷酸替换（14 个核苷酸；2 个空位）。与盐耐受新种 Cystobasidium halotolerans 不同，相关种 Cystobasidium slooffiae、Cystobasidium minutum 和 Cystobasidium fimetarium 无法同化 d-半乳糖、可溶性淀粉、半乳糖醇、甲醇或硝酸盐，并且在 37°C 下生长。这个未描述的种被命名为盐耐受新种 Cystobasidium halotolerans sp. nov.。模式株为 QCC/Y31/17（=MUCL057192=CGMCC2.5570）。
