布氏梨形孢囊梗向光性的测量

Measurement of Phototropism of the Sporangiophore of Phycomyces blakesleeanus.

作者信息

Corrochano Luis M, Galland Paul

机构信息

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain.

Fachbereich Biologie, Philipps-Universität Marburg, Marburg, Germany.

出版信息

Methods Mol Biol. 2019;1924:63-81. doi: 10.1007/978-1-4939-9015-3_7.

DOI:10.1007/978-1-4939-9015-3_7

PMID:30694468

Abstract

The giant sporangiophore, fruiting body, of the fungus Phycomyces blakesleeanus is a single cell that grows guided by several environmental signals, including light. The phototropic response has been investigated in detail. Three proteins, the components of a photoreceptor and transcription factor complex and a regulator of the signal transduction protein Ras, participate in the signal transduction pathway. We describe the basic methods for characterizing phototropic bending and the correlated elongation and rotation responses of the sporangiophore.

摘要

真菌布氏根霉的巨大孢子囊梗，即子实体，是一个单细胞，它在包括光在内的多种环境信号的引导下生长。对其向光性反应已进行了详细研究。三种蛋白质，即一种光感受器和转录因子复合物的组成成分以及信号转导蛋白Ras的调节因子，参与了信号转导途径。我们描述了表征孢子囊梗向光弯曲以及相关伸长和旋转反应的基本方法。