Department of Comparative Biomedicine and Food Science , University of Padua , Viale dell'Università , Legnaro , 35020 , Italy.
Department of Life and Environmental Sciences , Marche Polytechnic University , via Brecce Bianche , Ancona , 60131 , Italy.
Biomacromolecules. 2019 Mar 11;20(3):1375-1384. doi: 10.1021/acs.biomac.8b01750. Epub 2019 Feb 12.
The ability of peculiar iron oxide nanoparticles (IONPs) to evade the immune system was investigated in vivo. The nanomaterial was provided directly into the farming water of zebrafish ( Danio rerio) and the distribution of IONPs and the delivery of oxytetracycline (OTC) was studied evidencing the successful overcoming of the intestinal barrier and the specific and prolonged (28 days) organotropic delivery of OTC to the fish ovary. Noteworthy, no sign of adverse effects was observed. In fish blood, IONPs were able to specifically bind apolipoprotein A1 (Apo A1) and molecular modeling showed the structural analogy between the IONP@Apo A1 nanoconjugate and high-density lipoprotein (HDL). Thus, the preservation of the biological identity of the protein suggests a plausible explanation of the observed overcoming of the intestinal barrier, of the great biocompatibity of the nanomaterial, and of the prolonged drug delivery (benefiting of the lipoprotein transport route). The present study promises novel and unexpected stealth materials in nanomedicine.
研究了特殊氧化铁纳米粒子（IONPs）在体内逃避免疫系统的能力。将纳米材料直接提供给斑马鱼（Danio rerio）的养殖水中，并研究了 IONPs 的分布和土霉素（OTC）的递送，证明了成功克服了肠道屏障，并且将 OTC 特异性和长时间（28 天）递送到鱼卵巢。值得注意的是，没有观察到不良反应的迹象。在鱼血液中，IONPs 能够特异性结合载脂蛋白 A1（Apo A1），分子建模表明 IONP@Apo A1 纳米复合物与高密度脂蛋白（HDL）之间存在结构相似性。因此，蛋白质的生物特性得以保留，这表明观察到的肠道屏障的克服、纳米材料的高生物相容性以及延长的药物递送（得益于脂蛋白转运途径）具有合理的解释。本研究有望为纳米医学带来新颖且出乎意料的隐形材料。
